Inulin with a low degree of polymerization protects human umbilical vein endothelial cells from hypoxia/reoxygenation-induced injury

  • Carbohydr Polym. 2019 Jul 15;216:97-106. doi: 10.1016/j.carbpol.2019.03.016.
Xuexiang Cha  1 ,  Shengna Han  1 ,  Jian Yu  2 ,  Saiyang Zhang  1 ,  Shuang Yu  1 ,  Dongjun Fu  3 ,  Min Yao  4 ,  Lirong Zhang  5 ,  Guoqing Feng  6
Affiliations
  • 1. Department of Pharmacology, College of Basic Medicine, Zhengzhou University, 450001 Zhengzhou, China.
  • 2. Faculty of Advanced Life Science, Hokkaido University, 060-0810 Sapporo, Japan.
  • 3. New Drug Research & Development Center, School of Pharmaceutical Sciences, Zhengzhou University, 450001 Zhengzhou, China.
  • 4. Department of Pharmacology, College of Basic Medicine, Zhengzhou University, 450001 Zhengzhou, China; Faculty of Advanced Life Science, Hokkaido University, 060-0810 Sapporo, Japan.
  • 5. Department of Pharmacology, College of Basic Medicine, Zhengzhou University, 450001 Zhengzhou, China. Electronic address: [email protected].
  • 6. Department of Pharmacology, College of Basic Medicine, Zhengzhou University, 450001 Zhengzhou, China. Electronic address: [email protected].
PMID: 31047086 DOI: 10.1016/j.carbpol.2019.03.016
Abstract

Here, we identified inulin-type oligosaccharides with 3-13 degrees of polymerization from Morinda officinalis. Radical-scavenging assays showed that Inulins 4-7 had modest anti-oxidative effects. Inulins 4 and 5 dose-dependently increased human umbilical vein endothelial cell survival during hypoxia/re-oxygenation (H/R)-induced injury, and Inulin 5 promoted angiogenesis. Triplicate assays with the Affymetrix Human Transcriptome Array 2.0 showed that Inulin 5 exposure up-regulated genes associated with cell cycle progression, Apoptosis, DNA replication and repair, ubiquitin-mediated proteolysis, the mitogen-activated protein kinase pathway, and the phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)-signaling pathway. Flow cytometry, reverse transcription-quantitative polymerase chain reaction, and western blot experiments verified the microarray results and demonstrated that Inulin 5 influenced cell cycle progression and the PI3K-protein kinase B (PKB)-endothelial nitric oxide synthase (eNOS) pathway. Thus, inulin-type oligosaccharides from M. officinalis roots may protect against H/R-induced injury, primarily through an anti-oxidative effect, and promote angiogenesis by activating the PI3K-PKB-eNOS-signaling pathway.

Keywords
Angiogenesis; Anti-oxidation; Hypoxia/reoxygenation; Inulin; Morinda officinalis.
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