The Difference in Cytotoxic Activity between Two Optical Isomers of Gelsemine from Gelsemium elegans Benth. on PC12 Cells

  • Molecules. 2019 May 25;24(10):2004. doi: 10.3390/molecules24102004.
Li Lin  1 ,  Yan-Chun Liu  2  3 ,  Zhao-Ying Liu  4  5
Affiliations
  • 1. College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China. [email protected].
  • 2. College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China. [email protected].
  • 3. Hunan Engineering Technology Research Center of Veterinary Drugs, Hunan Agricultural University, Changsha 410128, China. [email protected].
  • 4. College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China. [email protected].
  • 5. Hunan Engineering Technology Research Center of Veterinary Drugs, Hunan Agricultural University, Changsha 410128, China. [email protected].
PMID: 31130596 DOI: 10.3390/molecules24102004
Abstract

Two optical isomers, +/- gelsemine (1, 2), together with one known compound were isolated from the whole plant of G. elegans. The structures of the separated constituents were elucidated on 1D and 2D (1H-1H COSY, HMBC, HSQC) NMR spectroscopy and high-resolution mass spectrometry (HRMS). The isolated Alkaloids were tested in vitro for cytotoxic potential against PC12 cells by the MTT assay. As a result, (+) gelsemine (compound 1) exhibited cytotoxic activity against PC12 cells with an IC50 value of 31.59 μM, while (-) gelsemine (compound 2) was not cytotoxic.

Keywords
Gelsemium elegans; PC12 cells; gelsemine; koumine.
Products