A Multibasic Cleavage Site in the Spike Protein of SARS-CoV-2 Is Essential for Infection of Human Lung Cells
- Mol Cell. 2020 May 21;78(4):779-784.e5. doi: 10.1016/j.molcel.2020.04.022.
- 1. Deutsches Primatenzentrum - Leibniz Institut für Primatenforschung, Göttingen, Germany. Electronic address: [email protected].
- 2. Deutsches Primatenzentrum - Leibniz Institut für Primatenforschung, Göttingen, Germany; Faculty of Biology and Psychology, University Göttingen, Göttingen, Germany.
- 3. Deutsches Primatenzentrum - Leibniz Institut für Primatenforschung, Göttingen, Germany; Faculty of Biology and Psychology, University Göttingen, Göttingen, Germany. Electronic address: [email protected].
The pandemic coronavirus SARS-CoV-2 threatens public health worldwide. The viral spike protein mediates SARS-CoV-2 entry into host cells and harbors a S1/S2 cleavage site containing multiple arginine residues (multibasic) not found in closely related animal coronaviruses. However, the role of this multibasic cleavage site in SARS-CoV-2 Infection is unknown. Here, we report that the cellular protease Furin cleaves the spike protein at the S1/S2 site and that cleavage is essential for S-protein-mediated cell-cell fusion and entry into human lung cells. Moreover, optimizing the S1/S2 site increased cell-cell, but not virus-cell, fusion, suggesting that the corresponding viral variants might exhibit increased cell-cell spread and potentially altered virulence. Our results suggest that acquisition of a S1/S2 multibasic cleavage site was essential for SARS-CoV-2 Infection of humans and identify Furin as a potential target for therapeutic intervention.