Zuojin Pill ameliorates chronic atrophic gastritis induced by MNNG through TGF-β1/PI3K/Akt axis

  • J Ethnopharmacol. 2021 May 10;271:113893. doi: 10.1016/j.jep.2021.113893.
Yuling Tong  1 ,  Ruilin Wang  2 ,  Xia Liu  2 ,  Miao Tian  2 ,  Yanling Wang  2 ,  Yanfei Cui  2 ,  Wenjun Zou  3 ,  Yanling Zhao  4
Affiliations
  • 1. College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China; Department of Pharmacy, Chinese PLA General Hospital, Beijing, China.
  • 2. Department of Pharmacy, Chinese PLA General Hospital, Beijing, China.
  • 3. College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China. Electronic address: [email protected].
  • 4. Department of Pharmacy, Chinese PLA General Hospital, Beijing, China. Electronic address: [email protected].
PMID: 33524511 DOI: 10.1016/j.jep.2021.113893
Abstract

Ethnopharmacological relevance: Zuojin Pill (ZJP) is a classic prescription composed of Coptis chinensis and Tetradium ruticarpum (A.Juss.) T.G.Hartley, which is often used in the treatment of digestive system diseases.

Aim of this study: The purpose of this study was to explore the therapeutic effect and potential mechanism of ZJP on chronic atrophic gastritis (CAG) induced by MNNG.

Materials and methods: The GES-1 and rat model of CAG was established by MNNG. Detection of cell viability, morphological changes and proliferation of GES-1 by CCK-8 and high content screening (HCS) assay. G-17, IL-8 and TNF-α in rat serum were detected by ELISA kit. The expression of related mRNA and protein on TGF-β1/PI3K/Akt signal axis were detected by RT-PCR and Western blot.

Results: The results showed that ZJP could significantly improve the GES-1 damage induced by MNNG and improve the gastric histomorphology of CAG rats. The intervention of ZJP could significantly reduce the content of G-17 and inflammatory factors IL-8, TNF- α, IL-6 and IL-1β, inhibit the expression of TGF-β1, PI3K and their downstream signals p-Akt, p-mTOR, p70S6K, and promote the expression level of PTEN, LC3-II and Beclin-1.

Conclusion: ZJP has a good therapeutic effect on CAG induced by MNNG, which may be closely related to the inhibition of TGF-β1/PI3K/Akt signal pathway.

Keywords
Chronic atrophic gastritis; Coptis chinensis; MNNG; TGF-β1/PI3K/Akt; Tetradium ruticarpum; Zuojin pill.
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