Manganese Mineralization of Pathogenic Viruses as a Universal Vaccine Platform

  • Adv Sci (Weinh). 2023 Oct 22:e2303615. doi: 10.1002/advs.202303615.
Pan-Deng Shi  1 ,  Yan-Peng Xu  2 ,  Zhu Zhu  1  3 ,  Chao Zhou  1 ,  Mei Wu  1 ,  Yangzhige He  4 ,  Hui Zhao  1 ,  Liying Liu  2 ,  Linqing Zhao  2 ,  Xiao-Feng Li  1 ,  Cheng-Feng Qin  1
Affiliations
  • 1. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, 100071, China.
  • 2. Laboratory of Virology, Beijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing, 100020, China.
  • 3. School of Medicine, Tsinghua University, Beijing, 100091, China.
  • 4. Department of Medical Research Center, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, 100730, China.
PMID: 37867242 DOI: 10.1002/advs.202303615
Abstract

Biomimetic viral mineralization improves viral vaccine stability and immunogenicity using inorganic metals such as CA, Al, or Fe. Mn is a metal found in high concentrations in mammalian tissues; however, under natural or laboratory conditions, Mn mineralization by medical viruses has yet to be established. Herein, a single IAV particle is successfully encapsulated with manganese phosphate (MnP) under specific conditions using the human influenza A virus (IAV). MnP-mineralized IAVs (IAV@Mn) exhibited physiochemical and in vitro properties similar to Ca-mineralized IAVs. In animal models, IAV@Mn shows limited replication in immune-competent cells and a significant attenuation compared to naïve cells. Moreover, a single-dose vaccination with IAV@Mn induced robust humoral and cellular immune responses and conferred significant protection against a wild-type IAV challenge in mice. Thus, Mn mineralization in pathogenic viruses provides a rapid and universal strategy for generating an emergency vaccine in response to emerging viruses.

Keywords
innate immune activation; manganese mineralization; novel vaccine; resist infection; virulence attenuation.
Products