Role of transglutaminase 2 in promoting biglycan synthesis in idiopathic gingival fibromatosis

  • BMC Oral Health. 2024 Nov 21;24(1):1422. doi: 10.1186/s12903-024-05211-8.
Yurika Ninomiya  #  1 ,  Shinji Matsuda  #  2 ,  Shigeki Suzuki  3  4 ,  Shizu Hirata-Tsuchiya  5 ,  Tomoya Ueda  1 ,  Fuminori Nakashima  1 ,  Keisuke Yasuda  1 ,  Shogo Shimada  1 ,  Takumi Memida  6 ,  Tetsuya Yoshimoto  7 ,  Satoru Yamada  3 ,  Kazuhisa Ouhara  1 ,  Noriyoshi Mizuno  1
Affiliations
  • 1. Department of Periodontal Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan.
  • 2. Department of Periodontal Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan. [email protected].
  • 3. Department of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai, Japan.
  • 4. Department of Operative Dentistry, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Sendai, Japan.
  • 5. Department of Biological Endodontics, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
  • 6. Department of Oral Science and Translation Research, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, USA.
  • 7. Center of Oral Clinical Examination, Hiroshima University Hospital, Hiroshima, Japan.
  • # Contributed equally.
PMID: 39574071 DOI: 10.1186/s12903-024-05211-8
Abstract

Background: This study aimed to elucidate the pathogenesis of idiopathic gingival fibromatosis (IGF).

Methods: Human gingival fibroblasts (hGFs) were isolated from patients with IGF and periodontitis. Differential gene expression in the hGFs was analyzed using RNA Sequencing. Extracellular matrix-related gene expression in the hGFs was analyzed. The effect of specific protein (SP)1 inhibitor or recombinant human Transglutaminase 2 (rh-TGM2) on biglycan (BGN) expression in hGFs was also determined.

Results: RNA Sequencing showed that TGM2 expression was downregulated and BGN mRNA expression was upregulated in patients with IGF relative to periodontitis. rh-TGM2 stimulation of hGFs in patients with IGF significantly reduced BGN expression. SP1 inhibitors downregulated BGN expression in the hGFs.

Conclusion: BGN upregulation via SP1 causes TGM2 downregulation in gingival fibroblasts in IGF.

Keywords
Biglycan; Human gingival fibroblast; Idiopathic gingival fibromatosis; Periodontitis; Specific protein 1; Transglutaminase 2.
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