Layer-Restacked 3D Ti3C2 Nanostructures with Efficient Photothermal Antibacterial Activities

  • ACS Appl Bio Mater. 2025 May 19;8(5):3824-3832. doi: 10.1021/acsabm.4c01997.
Jinnan Xuan  1  2 ,  Shuxian Hou  3 ,  Yuqiang Han  1 ,  Chen Li  2 ,  Yisi Liu  1 ,  Zhong Li  1 ,  Xixia Liu  4 ,  Guoqiang Yang  3 ,  Xinxin Liu  3 ,  Jiantao Wang  3 ,  Yuting Huang  5 ,  Jun Wang  3  6 ,  Wei Lai  7
Affiliations
  • 1. Hubei Key Laboratory of Photoelectric Materials and Devices, School of Materials Science and Engineering, Hubei Normal University, 11 Cihu Road, Huangshi 435002, P. R. China.
  • 2. Laboratory of Solid State Microstructures, Nanjing University, Nanjing 210093, P. R. China.
  • 3. College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, P. R. China.
  • 4. Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization; Hubei Engineering Research Center of Special Wild Vegetables Breeding and Comprehensive Utilization Technology, Hubei Normal University, 11 Cihu Road, Huangshi 435002, Hubei Province, P. R. China.
  • 5. Department of Radiotherapy, Chaohu Hospital of Anhui Medical University, 64 Chaohu North Road, Chaohu 238000, P. R. China.
  • 6. Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, P. R. China.
  • 7. Hubei Key Laboratory of Energy Storage and Power Battery, School of Mathematics, Physics and Optoelectronic Engineering, Hubei University of Automotive Technology, Shiyan 442002, P. R. China.
PMID: 40275489 DOI: 10.1021/acsabm.4c01997
Abstract

Multidrug-resistant Bacterial infections have emerged as a global public health crisis due to Antibiotic misuse. In this study, we develop a layer-restacked 3D Ti3C2 nanostructure utilizing ice-templating. This nanostructure exhibits outstanding hydrophilicity, biocompatibility, and stability, as well as enhanced absorption, extinction coefficient, and photothermal conversion efficiency. Additionally, the layer-restacked 3D Ti3C2 nanostructure demonstrates excellent Antibacterial activity against MDR Escherichia coli and MDR Staphylococcus aureus irradiated by 808 nm near-infrared light (NIR). Specifically, the mechanism of photothermal action against multidrug-resistant bacteria involves structural damage to the Bacterial membranes, leading to the leakage of Bacterial contents after layer-restacked 3D Ti3C2 nanostructures adhered under NIR irradiation. The results of transcriptome analysis show that the 3D Ti3C2 nanostructure regulates the membrane transporters and membrane transporter proteins on the Bacterial cell membrane as well as the activities of Enzymes associated with them, which in turn affect the metabolic processes of organic acids and Other organic substances in the Bacterial cell. The DNA-binding transcriptional activator EvgA is significantly downregulated, which may play a crucial role in inhibiting the emergence of drug resistance in bacteria when exposed to the layer-restacked 3D Ti3C2 nanostructure. The layer-restacked 3D Ti3C2 nanostructure is an effective photothermal antimicrobial nanostructure against multidrug-resistant bacteria.

Keywords
freeze-drying ice-template; layer-restacked 3D Ti3C2 nanostructures; multidrug-resistant bacteria; photothermal antibacterial activities; transcriptomic analysis.
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