Protocol for lentiviral generation and transduction of human-derived endometrial epithelial organoids to study uterine function and pathologies

  • STAR Protoc. 2026 Jun 19;7(2):104558. doi: 10.1016/j.xpro.2026.104558.
Sydney E Parks  1 ,  Diana Monsivais  2
Affiliations
  • 1. Department of Pathology & Immunology, Baylor College of Medicine, Houston, TX 77030, USA; Center for Drug Discovery, Baylor College of Medicine, Houston, TX 77030, USA. Electronic address: [email protected].
  • 2. Department of Pathology & Immunology, Baylor College of Medicine, Houston, TX 77030, USA; Center for Drug Discovery, Baylor College of Medicine, Houston, TX 77030, USA. Electronic address: [email protected].
PMID: 42101936 DOI: 10.1016/j.xpro.2026.104558
Abstract

The use of patient-derived endometrial epithelial organoids has revolutionized the field of uterine biology. Here, we present a protocol for generating lentivirus and transducing human-derived endometrial epithelial organoids for the study of endometrial function and pathologies. We describe steps for lentiviral production, lentiviral transduction of endometrial epithelial organoids, and endometrial epithelial Organoid passaging. We then detail procedures for validating lentiviral transduction. This protocol allows for knocking down or overexpressing genes of interest, enabling investigation of specific targets in a patient-centric model.

Keywords
Cell Biology; Cell culture; Gene Expression; Organoids.
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