CA5A Protein, Human (His-Met)

CA5A protein is a mitochondrial carbonic anhydrase that catalyzes the reversible conversion of carbon dioxide to bicarbonate/HCO3, providing essential bicarbonate for mitochondrial enzymes. Impermeable mitochondria rely on CA5A to support enzymatic reactions critical to intermediary metabolism, including the urea cycle and the Krebs cycle. CA5A Protein, Human (His-Met) is the recombinant human-derived CA5A protein, expressed by E. coli , with N-Met, C-His labeled tag. The total length of CA5A Protein, Human (His-Met) is 266 a.a., with molecular weight of ~33 kDa.

CA5A protein is a mitochondrial carbonic anhydrase crucial for the reversible conversion of carbon dioxide to bicarbonate/HCO3 within the mitochondria, as indicated by its catalytic activity. Given that mitochondria are impermeable to HCO3, the role of CA5A becomes pivotal in supplying bicarbonate for various mitochondrial enzymes. These enzymes are integral to the synthesis of essential metabolites in intermediary metabolism, particularly in processes such as the urea and Krebs cycles. The activity of CA5A underscores its significance in maintaining the metabolic integrity of mitochondrial functions by facilitating the availability of bicarbonate for key enzymatic reactions.

Measured by its esterase activity. The specific activity is >500 pmoles/min/μg.

Human

E. coli

C-His

P35218 (A40-S305)

763  [NCBI]

Approximately 33 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 50 mM NaAc, 50 mM NaCl, 0.05% Brij 35, pH 5.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.