clpB Protein, E.coli (K476C, His)
ClpB protein is a key component in the stress-induced multichaperone system, which cooperates with DnaK, DnaJ and GrpE to promote cell recovery from heat-induced injury. Upstream of DnaK, ClpB processes protein aggregates and upon binding stimulates their ATPase activity. clpB Protein, E.coli (K476C, His) is the recombinant E. coli-derived clpB protein, expressed by E. coli , with N-6*His labeled tag.
- Species: E.coli
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
ClpB protein is a key component in the stress-induced multichaperone system, which cooperates with DnaK, DnaJ and GrpE to promote cell recovery from heat-induced injury. Upstream of DnaK, ClpB processes protein aggregates and upon binding stimulates their ATPase activity. clpB Protein, E.coli (K476C, His) is the recombinant E. coli-derived clpB protein, expressed by E. coli , with N-6*His labeled tag.
ClpB operates as a pivotal component within a stress-induced multi-chaperone system, collaboratively orchestrating cellular recovery from heat-induced damage alongside DnaK, DnaJ, and GrpE. Acting upstream of DnaK, ClpB plays a crucial role in processing protein aggregates, with its ATPase activity stimulated upon protein binding. The ensuing ATP hydrolysis facilitates the unfolding of denatured protein aggregates, potentially exposing new hydrophobic binding sites on ClpB-bound aggregates. This orchestrated process contributes to the solubilization and refolding of denatured protein aggregates, an essential cooperative effort involving DnaK. In its functional state, ClpB forms a homohexamer, and the oligomerization is intricately regulated by ATP, underscoring the dynamic nature of its involvement in cellular stress responses.
Technical Parameters
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Species E.coli
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Source E. coli
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Tag N-6*His
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Accession
P63286 (M1-Q857, K476C)
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Gene ID/
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Molecular Construction
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N-term
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6*His
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clpB (M1-Q857, K476C)
Accession # P63286 -
C-term
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Protein Length
Full Length
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Synonyms
CLPB; Caseinolytic Peptidase B Protein Homolog; ClpB Family Mitochondrial Disaggregase; ClpB Caseinolytic Peptidase B Homolog; Suppressor Of Potassium Transport Defect 3; Testicular Secretory Protein Li 11; SKD3; CLPB Protein; Mitochondrial Disaggregase;
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AA Sequence
MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGGSVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRGDNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQALKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILFIDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQKVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAIDLIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSDKERQYSELEEEWKAEKASLSGTQTICAELEQAKIAIEQARRVGDLARMSELQYGKIPELEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQELHHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFDSDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKELVLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDEALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAVQ
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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