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hydB Protein, Pyrococcus furiosus

HydB is an important component of the bifunctional enzyme complex and is a NADPH-dependent hydrogen-evolving hydrogenase with sulfur reducing activity. It contributes to the hydrogen cycle during fermentative growth, with the beta and gamma subunits catalyzing the cytoplasmic production of hydrogen sulfide from elemental sulfur. hydB Protein, Pyrococcus furiosus is the recombinant hydB protein, expressed by E. coli , with tag free.

For research use only. We do not sell to patients.
  • Species: Others
  • Source: E. coli
  • hydB Protein, Pyrococcus furiosus Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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Biological Activity

Description

HydB is an important component of the bifunctional enzyme complex and is a NADPH-dependent hydrogen-evolving hydrogenase with sulfur reducing activity. It contributes to the hydrogen cycle during fermentative growth, with the beta and gamma subunits catalyzing the cytoplasmic production of hydrogen sulfide from elemental sulfur. hydB Protein, Pyrococcus furiosus is the recombinant hydB protein, expressed by E. coli , with tag free.

Background

HydB is a component of a bifunctional enzyme complex, acting as an NADPH-dependent hydrogen-evolving hydrogenase with sulfur reducing activity. This enzyme may contribute to hydrogen cycling during fermentative growth. Notably, its activity is not demonstrated with NAD, and the beta and gamma subunits together form the sulfur-reducing component responsible for catalyzing the cytoplasmic production of hydrogen sulfide in the presence of elemental sulfur. However, HydB is inactive when exposed to sodium sulfate, sodium sulfite, sodium thiosulfate, or cysteine. These findings highlight its role in the intricate processes of hydrogen metabolism and sulfur reduction, specifically under certain environmental conditions.

Technical Parameters

  • Species Others
  • Source E. coli
  • Tag Tag Free
  • Accession

    Q8U2E5 (M1-V367)

  • Gene ID
    41712699
  • Molecular Construction
    • N-term
    • hydB (M1-V367)
      Accession # Q8U2E5
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    Sulfhydrogenase 1 subunit beta; EC:1.12.98.4; Sulfhydrogenase I subunit beta; Sulfur reductase subunit HydB; Sulfur reductase subunit Hyd; hydB; PF0891

  • AA Sequence

    MRYVKLPKENTYEFLERLKDWGKLYAPVKISDKFYDFREIDDVRKIEFHYNRTIMPPKKFFFKPREKLFEFDISKPEYREVIEEVEPFIIFGVHACDIYGLKILDTVYLDEFPDKYYKVRREKGIIIGISCMPDEYCFCNLRETDFADDGFDLFFHELPDGWLVRVGTPTGHRLVDKNIKLFEEVTDKDICAFRDFEKRRQQAFKYHEDWGNLRYLLELEMEHPMWDEEADKCLACGICNTTCPTCRCYEVQDIVNLDGVTGYRERRWDSCQFRSHGLVAGGHNFRPTKKDRFRNRYLCKNAYNEKLGLSYCVGCGRCTAFCPANISFVGNLRRILGLEENKCPPTVSEEIPKRGFAYSSNIRGDGV

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Calculators

Reconstitution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
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Biological Activity (ED50) Biological Activity (ED50)
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ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • hydB Protein, Pyrococcus furiosus Product Image