Integrin beta-1/CD29 Protein, Mouse (His)

Integrin beta-1/CD29 is one of the most common subunits in the integrin family. Integrin beta-1/CD29 functions as a component of a heterodimeric complex in cells, which forms an adhesion receptor with the α-integrin subunit, mediating cell signaling. Integrin beta-1/CD29 mediates cell-to-cell and cell-to-extracellular matrix interactions, participating in embryogenesis and development, differentiation and migration, inflammatory responses, hematopoietic stem cell differentiation, supramolecular assembly of extracellular matrix proteins, and tumor progression. Integrin beta-1/CD29 Protein, Mouse (His), is a recombinant Integrin beta-1/CD29 protein with a N-10*His tag^[1][2][3][4].

Integrin beta-1/CD29 is a multifunctional heterodimeric glycoprotein widely involved in various physiological and pathological processes. Integrin beta-1/CD29 is highly expressed in dermal fibroblasts and articular chondrocytes, located in adhesion focal structures, and mediates cell adhesion to the extracellular matrix, including collagen, fibronectin, and laminin. Integrin beta-1/CD29 also connects actin stress fibers in myofibroblasts to the extracellular matrix, providing structural support for cellular mechanotransmission. Integrin beta-1/CD29 can activate latent TGF-β through mechanotransmission, making it a core regulatory molecule for skin wound healing. Simultaneously, Integrin beta-1/CD29 positively regulates the synthesis of type I collagen and α-smooth muscle actin. Animal experiments have shown that knockout mice are resistant to bleomycin-induced skin fibrosis and exhibit a progressive thinning of the dermis. Integrin beta-1/CD29 regulates the expression of extracellular matrix-related genes via the FAK/rac1/NOX/ROS pathway; its deletion significantly inhibits FAK phosphorylation, rac1 activity, and ROS generation. Integrin beta-1/CD29 can maintain the normal arrangement of the chondrocyte cytoskeleton, mediate cell signal transduction and intercellular communication, and play a role in stabilizing chondrocyte phenotype, inhibiting cell apoptosis, and regulating the expression of chondrocyte-specific genes ^[1][2][3][4].

Integrin beta-1/CD29 Protein, Mouse (2 h incubation) is used as antigen in Western blot assay, and it is specifically recognized by MAb GP1A5, GP1A6 and GP4A1, presenting a single protein band at 30 kDa^[1].

Mouse

E. coli

N-10*His

P09055 (Q21-K798)

16412

Full Length of Mature Protein

QTDKNRCLKANAKSCGECIQAGPNCGWCTNTTFLQEGMPTSARCDDLEALKKKGCQPSDIENPRGSQTIKKNKNVTNRSKGMAEKLRPEDITQIQPQQLLLKLRSGEPQKFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTDRGEFFNELVGQQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNVYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSGNSSNVIQLIIDAYNSLSSEVILENSKLPDGVTINYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITANKCPNKESETIKIKPLGFTEEVEVVLQFICKCNCQSHGIPASPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCRCRVCECYPNYTGSACDCSLDTGPCLASNGQICNGRGICECGACKCTDPKFQGPTCETCQTCLGVCAEHKECVQCRAFNKGEKKDTCAQECSHFNLTKVESREKLPQPVQVDPVTHCKEKDIDDCWFYFTYSVNGNNEAIVHVVETPDCPTGPDIIPIVAGVVAGIVLIGLALLLIWKLLMIIHDRREFAKFEKEKMNAKWDTGENPIYKSAVTTVVNPKYEGK

≥ 85%, as determined by reducing SDS-PAGE.

• [1]. Paños G, et al. Analysis of Swine β1 Integrin (CD29) Epitopes Through Monoclonal Antibodies Developed Using Two Immunization Strategies. Hybridoma and Hybridomics. 2004 Oct;23(5):271-278.

  [2]. Sun L, et al. The characteristics and the multiple functions of integrin β1 in human cancers. J Transl Med. 2023 Nov 6;21(1):787.

  [3]. Leask A. Integrin β1: A Mechanosignaling Sensor Essential for Connective Tissue Deposition by Fibroblasts. Adv Wound Care (New Rochelle). 2013 May;2(4):160-166.

  [4]. Shakibaei M, et al. Diverse roles of integrin receptors in articular cartilage. Adv Anat Embryol Cell Biol. 2008;197:1-60.