1. Recombinant Proteins
  2. Enzymes & Regulators
  3. LpxC Protein, E.coli (His)

LpxC Protein, E.coli (His)

Cat. No.: HY-P71488
COA Handling Instructions

LpxC protein serves as a key enzyme in lipid A biosynthesis by catalyzing the hydrolysis of UDP-3-O-myristoyl-N-acetylglucosamine, marking a key and crucial step in this important pathway. Through this enzymatic process, LpxC promotes the conversion of its substrate into UDP-3-O-myristoylglucosamine and acetate, making a significant contribution to lipid A biosynthesis. LpxC Protein, E.coli (His) is the recombinant E. coli-derived LpxC protein, expressed by E. coli , with N-His labeled tag. The total length of LpxC Protein, E.coli (His) is 305 a.a., with molecular weight of ~38.0 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

LpxC protein serves as a key enzyme in lipid A biosynthesis by catalyzing the hydrolysis of UDP-3-O-myristoyl-N-acetylglucosamine, marking a key and crucial step in this important pathway. Through this enzymatic process, LpxC promotes the conversion of its substrate into UDP-3-O-myristoylglucosamine and acetate, making a significant contribution to lipid A biosynthesis. LpxC Protein, E.coli (His) is the recombinant E. coli-derived LpxC protein, expressed by E. coli , with N-His labeled tag. The total length of LpxC Protein, E.coli (His) is 305 a.a., with molecular weight of ~38.0 kDa.

Background

LpxC is an enzyme that plays a crucial role in lipid A biosynthesis by catalyzing the hydrolysis of UDP-3-O-myristoyl-N-acetylglucosamine. This reaction results in the formation of UDP-3-O-myristoylglucosamine and acetate, representing the committed step in the biosynthesis of lipid A, a key component of bacterial lipopolysaccharides. Lipid A is an essential structural element in the outer membrane of Gram-negative bacteria, contributing to membrane integrity and playing a role in host-pathogen interactions. LpxC's catalytic activity is pivotal for the production of lipid A, and the enzyme serves as a potential target for the development of antibacterial agents aimed at disrupting the synthesis of crucial bacterial cell wall components.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

E.coli

Source

E. coli

Tag

N-His

Accession

P0A725 (1M-305A)

Gene ID

58460764  [NCBI]

Molecular Construction
N-term
His
LpxC (1M-305A)
Accession # P0A725
C-term
Synonyms
lpxC; asmB; envA; b0096; JW0094; UDP-3-O-acyl-N-acetylglucosamine deacetylase; UDP-3-O-acyl-GlcNAc deacetylase; EC 3.5.1.108; Protein EnvA; UDP-3-O-[R-3-hydroxymyristoyl]-N-acetylglucosamine deacetylase
AA Sequence

MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDLNPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIPIMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTIDFNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVVDDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVLAKQEAWEYVTFQDDAELPLAFKAPSAVLA

Molecular Weight

Approximately 38.0 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against solution in Tris-based buffer, 50% glycerol.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

LpxC Protein, E.coli (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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LpxC Protein, E.coli (His)
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HY-P71488
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