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  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Protease 7/OmpT Protein, E.coli (His)

Protease 7/OmpT Protein, E.coli (His)

Cat. No.: HY-P71483
COA Handling Instructions

Protease 7/OmpT protein is a multifunctional enzyme that can cleave a variety of substrates, such as T7 RNA polymerase, ferric enterobactin receptor protein (FEP), and the antimicrobial peptide protamine. Its unique specificity for paired basic residues suggests a preference for specific amino acid configurations. Protease 7/OmpT Protein, E.coli (His) is the recombinant E. coli-derived Protease 7/OmpT protein, expressed by E. coli , with N-6*His labeled tag. The total length of Protease 7/OmpT Protein, E.coli (His) is 297 a.a., with molecular weight of ~37.5 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

Protease 7/OmpT protein is a multifunctional enzyme that can cleave a variety of substrates, such as T7 RNA polymerase, ferric enterobactin receptor protein (FEP), and the antimicrobial peptide protamine. Its unique specificity for paired basic residues suggests a preference for specific amino acid configurations. Protease 7/OmpT Protein, E.coli (His) is the recombinant E. coli-derived Protease 7/OmpT protein, expressed by E. coli , with N-6*His labeled tag. The total length of Protease 7/OmpT Protein, E.coli (His) is 297 a.a., with molecular weight of ~37.5 kDa.

Background

Protease 7/OmpT Protein is a versatile enzyme with the capability to cleave various substrates, including T7 RNA polymerase, ferric enterobactin receptor protein (FEP), antimicrobial peptide protamine, and other proteins. Notably, this protease demonstrates a distinct specificity for paired basic residues, reflecting its preference for particular amino acid configurations. The ability of Protease 7/OmpT to target a range of proteins, coupled with its specific recognition of paired basic residues, underscores its significance in cellular processes and highlights its potential as a molecular tool in biotechnological applications.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

E.coli

Source

E. coli

Tag

N-6*His

Accession

P09169 (21S-317F)

Gene ID

945185  [NCBI]

Molecular Construction
N-term
6*His
OmpT (21S-317F)
Accession # P09169
C-term
Synonyms
ompT; b0565; JW0554; Protease 7; EC 3.4.23.49; Omptin; Outer membrane protein 3B; Protease A; Protease VII
AA Sequence

STETLSFTPDNINADISLGTLSGKTKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSGWVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRVTNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF

Molecular Weight

Approximately 37.5 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl,1 mM EDTA, 6% Trehalose, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Protease 7/OmpT Protein, E.coli (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Protease 7/OmpT Protein, E.coli (His)
Cat. No.:
HY-P71483
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