UBCH7 Protein, Mouse (Active, His)
UBCH7 Protein, Mouse (Active, His) is the recombinant mouse-derived UBCH7, expressed by E. coli , with His labeled tag. ,
- Species: Mouse
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
UBCH7 Protein, Mouse (Active, His) is the recombinant mouse-derived UBCH7, expressed by E. coli , with His labeled tag. ,
UBCH7 is a ubiquitin-conjugating enzyme E2 that specifically functions with HECT-type and RBR family E3 ubiquitin-protein ligases. Unlike most RING-containing E3 ligases, it lacks intrinsic E3-independent reactivity with lysine but exhibits activity with RBR family E3 enzymes (e.g., PRKN, RNF31, ARIH1), which operate like RING-HECT hybrids. It accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to target proteins. By similarity, it mediates ubiquitination by the CUL9-RBX1 complex. In vitro, it catalyzes 'Lys-11'-linked polyubiquitination and participates in the selective degradation of short-lived or abnormal proteins. Downregulated during the S-phase, it contributes to cell cycle progression. Additionally, it regulates nuclear hormone receptor transcriptional activity and may play a role in myelopoiesis.
Technical Parameters
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Species Mouse
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Source E. coli
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Tag His
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Accession
P68037 (M1-D154)
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Gene ID22195
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Protein Length
Full Length
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Synonyms
UBE2L3; Ubiquitin Conjugating Enzyme E2 L3; UBCH7; Ubiquitin‑Conjugating Enzyme E2 L3; E2 Ubiquitin‑Conjugating Enzyme L3; Ubiquitin‑Conjugating Enzyme E2‑F1; Ubiquitin Carrier Protein L3; Ubiquitin‑Protein Ligase L3; L‑UBC; Ubiquitin‑Conjugating Enzyme E
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AA Sequence
MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD
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Predicted Molecular Mass
20.4 kDa
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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