96314-96-4
Chemical Structure
INDO 1
- CAS No.: 96314-96-4
- Formula:C32H31N3O12
- Molecular Weight:649.60
IUPAC Name: 2,2'-((2-(2-(2-(bis(carboxymethyl)amino)-5-(6-carboxy-1H-indol-2-yl)phenoxy)ethoxy)-4-methylphenyl)azanediyl)diacetic acid
InChIKey: AMHAQOBUZCQMHN-UHFFFAOYSA-N
SMILES: O=C(C1=CC2=C(C=C1)C=C(C3=CC=C(N(CC(O)=O)CC(O)=O)C(OCCOC4=CC(C)=CC=C4N(CC(O)=O)CC(O)=O)=C3)N2)O
Biological Activity: INDO 1 is a cell-impermeable, dual-emission ratiometric fluorescent calcium indicator with a KD·β value of 0.44 μM. INDO 1 binds to intracellular free calcium ions and undergoes wavelength-dependent fluorescence changes. In the unbound state (free form) without Ca2+, its fluorescence emission peak is at 485 nm upon excitation with ultraviolet light (350 nm). When INDO 1 pentasodium binds to Ca2+ (bound state), the emission peak shifts to 405 nm (Ex = 350 nm; dual emission at Em= 405/485 nm)[1][2][3].
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INDO 1 | INDO 1 is a cell-impermeable, dual-emission ratiometric fluorescent calcium indicator with a KD·β value of 0.44 μM. INDO 1 binds to intracellular free calcium ions and undergoes wavelength-dependent fluorescence changes. In the unbound state (free form) without Ca2+, its fluorescence emission peak is at 485 nm upon excitation with ultraviolet light (350 nm). When INDO 1 pentasodium binds to Ca2+ (bound state), the emission peak shifts to 405 nm (Ex = 350 nm; dual emission at Em= 405/485 nm). | |||||||||||||||||||||
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- [1]. Hardie RC, et al. INDO-1 measurements of absolute resting and light-induced Ca2+ concentration in Drosophila photoreceptors. The Journal of neuroscience : the official journal of the Society for Neuroscience. 1996 May 01;16(9):2924-33.
- [2]. Hove-Madsen L, et al. Indo-1 binding to protein in permeabilized ventricular myocytes alters its spectral and Ca binding properties. Biophysical journal. 1992 Jul;63(1):89-97.
- [3]. Jacquemond V, et al. Indo-1 fluorescence signals elicited by membrane depolarization in enzymatically isolated mouse skeletal muscle fibers. Biophysical journal. 1997 Aug;73(2):920-8.
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