1. Metabolic Enzyme/Protease
    Anti-infection
  2. HCV Protease
    HCV

ACH-806 (Synonyms: GS9132)

Cat. No.: HY-19512
Handling Instructions

ACH-806 is an NS4A antagonist which can inhibit Hepatitis C Virus (HCV) replication with an EC50 of 14 nM.

For research use only. We do not sell to patients.

ACH-806 Chemical Structure

ACH-806 Chemical Structure

CAS No. : 870142-71-5

Size Price Stock
1 mg USD 751 Get quote
5 mg USD 1202 Get quote
10 mg USD 1923 Get quote

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

ACH-806 is an NS4A antagonist which can inhibit Hepatitis C Virus (HCV) replication with an EC50 of 14 nM.

IC50 & Target

NS4A[1]
EC50: 14 nM (HCV)[1]

In Vitro

ACH-806 is an NS4A antagonist which can inhibit Hepatitis C Virus (HCV) replication with an EC50 of 14 nM. ACH-806 treatment results in significant reductions of both NS3 and NS4A in the transfected cells. This finding is reminiscent of ACH-806-treated replicon cells in which the amounts of NS3 and NS4A are also both decreased. The total amount of NS3 in the ACH-806-treated sample is reduced by ~6-fold (100/16) and causes a reduction of NS4A-bound NS3 ~29-fold (261/9). The levels of labeled NS3 and NS4A immunoprecipitated by anti-NS3 antibody are apparently reduced after the treatment of ACH-806. ACH-806 also induces significant decreases of NS3 and NS4A and promotes p14 formation in the parental replicon cells but not in the ACH-806-resistant replicon cells[1].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 2.4305 mL 12.1524 mL 24.3049 mL
5 mM 0.4861 mL 2.4305 mL 4.8610 mL
10 mM 0.2430 mL 1.2152 mL 2.4305 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay
[1]

Huh-luc/neo cells are seeded in 96-well plates at a density of 8000 cells per well in a final volume of 200 μL of Dulbecco modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum. One day after seeding, ACH-806 is serially diluted in 100% dimethyl sulfoxide (DMSO) and added to cells at a 1:200 dilution, achieving a final concentration of 0.5% DMSO in a total volume of 200 μL. Cells are further incubated for 3 days (96 h post-seeding), and the inhibition of HCV replicon replication is quantified by measurement of luciferase activity using a commercial kit[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

411.44

Formula

C₁₉H₂₀F₃N₃O₂S

CAS No.

870142-71-5

SMILES

O=C(C1=CC=CN=C1)NC(NC2=CC=C(OCCCCC)C(C(F)(F)F)=C2)=S

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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Product Name:
ACH-806
Cat. No.:
HY-19512
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