Gap 27

Name: Gap 27
Brand: MedChemExpress
SKU: HY-P0139
Rating: 5.0 (7 reviews)

Cat. No.: HY-P0139 Purity: 99.90%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Gap 27, a synthetic connexin43 mimetic peptide, is a gap junction inhibitor. Gap 27 possesses conserved sequence homology to a portion of the second extracellular loop leading into the fourth transmembrane connexin segment.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

CAS No. : 198284-64-9

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	Get quote
200 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 7 publication(s) in Google Scholar

7 Publications Citing Use of MCE Gap 27

•bioRxiv. 2023 Nov 9.

In Vivo Efficacy Study

RT-PCR

: Gap 27 purchased from MedChemExpress. Usage Cited in: Brain Behav Immun. 2026 Jan:131:106176. [Abstract]; Gap 27 (1 mg/mL; 1 μL bilaterally microinjected into the medial prefrontal cortex (mPFC) over 10 min) significantly decreased Cx43 expression levels in the mPFC of mice, whereas RBD administration did not further alter Cx43 expression.

: Gap 27 purchased from MedChemExpress. Usage Cited in: Brain Behav Immun. 2026 Jan:131:106176. [Abstract]; Gap 27 (1 mg/mL; 1 μL bilaterally microinjected into the medial prefrontal cortex (mPFC) over 10 min) pretreatment markedly decreased sucrose preference in the sucrose preference test (SPT), increased immobility time in the forced swim test (FST), reduced locomotor activity in the open field test (OFT), and prolonged immobility time in the tail suspension test (TST) compared to control mice.

: Gap 27 purchased from MedChemExpress. Usage Cited in: Brain Behav Immun. 2026 Jan:131:106176. [Abstract]; Gap 27 (1 mg/mL; 1 μL bilaterally microinjected into the medial prefrontal cortex (mPFC) over 10 min) significantly reduced the proportion of Cx43 fluorescent spots at the interstitial junctions of astrocytes in the medial prefrontal cortex of mice, while RBD treatment had no such effect.

: Gap 27 purchased from MedChemExpress. Usage Cited in: Brain Behav Immun. 2026 Jan:131:106176. [Abstract]; Representative immunoblots of the cGAS-STING signaling pathway in primary astrocytes in the Vehicle, Gap 27 (200 μM; 24 h), and Gap 27 + RBD (10 nM; 24 h) groups were shown. The results showed that Gap 27 treatment significantly increased protein levels of cGAS and activated components of the cGAS-STING pathway, including p-STING/STING, p-TBK1/TBK1, and p-IRF3/IRF3. Notably, these protein levels remained unchanged when RBD was added to Gap 27-treated astrocytes.

: Gap 27 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2023 Jun;39(3):679-702. [Abstract]; The PWT and PWL of mice after intrathecal injection of Gap27 (14.4, 43.2, 144 nM; 10 μL) for 2 h, compared with the VCR group at the same point in time, n = 8 mice/group. The results indicated that, compared with the VCR group at the same time point, intrathecal injections of Gap27 dose-dependently reversed mechanical allodynia and heat hyperalgesia at 2 h after treatment.

: Gap 27 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2023 Jun;39(3):679-702. [Abstract]; Western blotting and quantitative analysis of Cx43 protein expression in spinal cord of mice treated with Gap 27 (14.4, 43.2, 144 nM; 10 μL; intrathecal injection), n = 3 mice/group. The results suggested that the protein levels of Cx43 declined remarkably after the first intrathecal injection of Gap 27 for 36 h.

: Gap 27 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2023 Jun;39(3):679-702. [Abstract]; qRT-PCR analysis of spinal cord Cx43 mRNA level after Gap 27 (14.4, 43.2, 144 nM; 10 μL; intrathecal injection) injection, n = 4 mice/group. The results suggested that the mRNA levels of Cx43 declined remarkably after the first intrathecal injection of Gap27 for 36 h.

: Gap 27 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2023 Jun;39(3):679-702. [Abstract]; Immunofluorescence images of Cx43 intensity in the astrocyte cultures following treatment with Gap 27 (30 μM) for 24 h, n = 3 cultures/group. The results showed that VCR treatment (3 nM, 24 h) evoked a pronounced upregulation in the fluorescence intensity of astrocytes Cx43 and this was reversed by Gap 27 (30 μM).

: Gap 27 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2023 Jun;39(3):679-702. [Abstract]; The Western blotting analysis proved that the increase in Cx43 expression induced by VCR was reduced by 41.2% in astrocyte cultures treated with Gap 27 (30 μM) for 24 h.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

In Vitro

Gap 27 causes a remarked decrease in the number of both TRAP-positive mononuclear and multinucleated rat osteoclasts cultured on bovine bone slices. The activity of the remaining osteoclasts is found to be diminished by measuring the percentage of osteoclasts with actin rings of all TRAP-positive cells. In addition, the resorbed area in the treated cultures is greatly diminished^[1].
Incubation of the carotid artery with the gap junction inhibitor Gap 27 (500 μM) essentially abolishes the hyperpolarization to acetylcholine but it is without effect on that to levcromakalim. In the guinea-pig isolated internal carotid artery, Gap 27 inhibits acetylcholine-induced, endothelium-dependent hyperpolarizations^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Gap 27 (300 μM) inhibits relaxation by 40% in thoracic aorta and the superior mesenteric artery. Gap 27 also attenuates the endothelium-dependent component of the relaxation induced by ATP in thoracic aorta. ^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1304.53

Formula

C₆₀H₁₀₁N₁₅O₁₇

CAS No.

198284-64-9

Appearance

Solid

Color

White to off-white

Sequence

Ser-Arg-Pro-Thr-Glu-Lys-Thr-Ile-Phe-Ile-Ile

Sequence Shortening

SRPTEKTIFII

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : ≥ 33.33 mg/mL (25.55 mM)

DMSO : 22.22 mg/mL (17.03 mM; ultrasonic and adjust pH to 2 with 1 M HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.7666 mL	3.8328 mL	7.6656 mL
5 mM	0.1533 mL	0.7666 mL	1.5331 mL
10 mM	0.0767 mL	0.3833 mL	0.7666 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (276 KB) SDS (254 KB)

COA (249 KB) RP-HPLC (253 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Ilvesaro J, et al. Connexin-mimetic peptide Gap 27 decreases osteoclastic activity. BMC Musculoskelet Disord. 2001;2:10. [Content Brief]

[2]. Edwards G, et al. Role of gap junctions in the responses to EDHF in rat and guinea-pig small arteries. Br J Pharmacol. 1999 Dec;128(8):1788-94. [Content Brief]

[3]. Chaytor AT,e t al. Central role of heterocellular gap junctional communication in endothelium-dependent relaxations of rabbit arteries. J Physiol. 1998 Apr 15;508 ( Pt 2):561-73. [Content Brief]

Cell Assay
^[1]

Bone cell cultures are cultured for 48 hours with three different treatments (control, heptanol and Gap 27). After the culture period, bone slices are fixed. The cells are stained for tartrate-resistant acid phosphatase (TRAP). To visualise the nuclei, the cells are incubated with the DNA-binding fluorochrome Hoechst 33258 (1 mg/mL stock diluted 1:800 in PBS) for 10 minutes at room temperature. The numbers of mononuclear and multinucleated TRAP-positive cells on each bone slice are counted^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Ilvesaro J, et al. Connexin-mimetic peptide Gap 27 decreases osteoclastic activity. BMC Musculoskelet Disord. 2001;2:10. [Content Brief]

[2]. Edwards G, et al. Role of gap junctions in the responses to EDHF in rat and guinea-pig small arteries. Br J Pharmacol. 1999 Dec;128(8):1788-94. [Content Brief]

[3]. Chaytor AT,e t al. Central role of heterocellular gap junctional communication in endothelium-dependent relaxations of rabbit arteries. J Physiol. 1998 Apr 15;508 ( Pt 2):561-73. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	0.7666 mL	3.8328 mL	7.6656 mL	19.1640 mL
	5 mM	0.1533 mL	0.7666 mL	1.5331 mL	3.8328 mL
	10 mM	0.0767 mL	0.3833 mL	0.7666 mL	1.9164 mL
	15 mM	0.0511 mL	0.2555 mL	0.5110 mL	1.2776 mL
H₂O	20 mM	0.0383 mL	0.1916 mL	0.3833 mL	0.9582 mL
H₂O	25 mM	0.0307 mL	0.1533 mL	0.3066 mL	0.7666 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Gap 27 Related Classifications

Cardiovascular Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Gap 27198284-64-9Gap27Gap-27Gap Junction ProteinInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Gap 27

Customer Review

Gap 27 Related Antibodies

7 Publications Citing Use of MCE Gap 27

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Gap 27 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Gap 27 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report