1. Cytoskeleton
  2. Gap Junction Protein
  3. Gap 27

Gap 27 

Cat. No.: HY-P0139 Purity: 98.34%
Handling Instructions

Gap 27, connexin43 mimetic peptide, is a gap junction inhibitor.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Gap 27 Chemical Structure

Gap 27 Chemical Structure

CAS No. : 198284-64-9

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1 mg USD 84 In-stock
Estimated Time of Arrival: December 31
5 mg USD 156 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

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Gap 27, connexin43 mimetic peptide, is a gap junction inhibitor.

In Vitro

Gap 27 causes a remarked decrease in the number of both TRAP-positive mononuclear and multinucleated rat osteoclasts cultured on bovine bone slices. The activity of the remaining osteoclasts is found to be diminished by measuring the percentage of osteoclasts with actin rings of all TRAP-positive cells. In addition, the resorbed area in the treated cultures is greatly diminished[1]. Incubation of the carotid artery with the gap junction inhibitor Gap 27 (500 μM) essentially abolishes the hyperpolarization to acetylcholine but it is without effect on that to levcromakalim. In the guinea-pig isolated internal carotid artery, Gap 27 inhibits acetylcholine-induced, endothelium-dependent hyperpolarizations[2].

In Vivo

Gap 27 (300 μM) inhibits relaxation by 40% in thoracic aorta and the superior mesenteric artery. Gap 27 also attenuates the endothelium-dependent component of the relaxation induced by ATP in thoracic aorta. [3].

Molecular Weight








Sequence Shortening



Room temperature in continental US; may vary elsewhere.

Powder -80°C 2 years
  -20°C 1 year
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 33.33 mg/mL (25.55 mM; Need ultrasonic)

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.7666 mL 3.8328 mL 7.6656 mL
5 mM 0.1533 mL 0.7666 mL 1.5331 mL
10 mM 0.0767 mL 0.3833 mL 0.7666 mL
*Please refer to the solubility information to select the appropriate solvent.
Cell Assay

Bone cell cultures are cultured for 48 hours with three different treatments (control, heptanol and Gap 27). After the culture period, bone slices are fixed. The cells are stained for tartrate-resistant acid phosphatase (TRAP). To visualise the nuclei, the cells are incubated with the DNA-binding fluorochrome Hoechst 33258 (1 mg/mL stock diluted 1:800 in PBS) for 10 minutes at room temperature. The numbers of mononuclear and multinucleated TRAP-positive cells on each bone slice are counted[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2


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