Tenatoprazole sodium

The enzyme suspension (20 mg/mL) is incubated at 37°C in a buffer composed of 5 mM Pipes/Tris (pH 6.95), 2 mM MgCl₂, 150 mM KCl, 3 mg/mL of valinomycin, 1 mM acridine orange, in the presence of 0.1 mM glutathione, in a SPEX spectrofluorometer for 5 min. Inhibitor (20 mM), omeprazole or tenatoprazole, is added and fluorescence is measured by excitation at 490 nm and emission at 530 nm. After 60 s, ATP (2 mM) is added to initiate acridine orange uptake as a measure of intra-vesicular acidification^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Rats: (R,S)-Tenatoprazole free form (50 mg/kg) or (S)-tenatoprazole sodium salt hydrate (50 mg/kg) is orally administered to dogs and the plasma level of tenatoprazole is measured as a function of time course. Similarly, (S)-tenatoprazole free form (100 mg/kg) or (S)-tenatoprazole sodium salt hydrate (100 mg/kg) is orally administered to dogs and the plasma level of tenatoprazole is measured as a function of time^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.