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  4. AAPDNRETF

AAPDNRETF is a dominant minor histocompatibility antigen presented by H-2Db， which antigen is expressed in C57BL/6 mice and can be recognized by T cells from C3H.SW mice, thereby inducing a strong immune response. AAPDNRETF can induce graft-versus-host disease in irradiated C57BL/6 recipient mice via transfer of sensitized T lymphocytes. AAPDNRETF is applicable to the research of graft-versus-host disease.

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AAPDNRETF

AAPDNRETF Chemical Structure

CAS No. : 179953-98-1

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AAPDNRETF Related Antibodies

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Description

AAPDNRETF is a dominant minor histocompatibility antigen presented by H-2Db， which antigen is expressed in C57BL/6 mice and can be recognized by T cells from C3H.SW mice, thereby inducing a strong immune response. AAPDNRETF can induce graft-versus-host disease in irradiated C57BL/6 recipient mice via transfer of sensitized T lymphocytes. AAPDNRETF is applicable to the research of graft-versus-host disease[1].

In Vitro

AAPDNRETF (0.01 nM-1 μM) effectively sensitizes target cells expressing H-2Db(e.g., T2Db cells) at an extremely low concentration (10 pM), rendering them susceptible to killing by mouse effector T cells (CTL)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AAPDNRETF Related Antibodies
In Vivo

AAPDNRETF (50-300 µg/mouse; s.c.; administered twice at 7-day intervals) induces a robust, specific cytotoxic T-lymphocyte response against the dominant C57BL/6 minor histocompatibility antigen presented by H-2Db in male C3H.SW/SnJ mice[1].
AAPDNRETF (300 µg/mouse; s.c.; administered twice at 7-day intervals) induces T lymphocytes that cause severe non-lethal graft-versus-host disease and thymic hypoplasia in irradiated male C57BL/6J recipient mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C3H.SW/SnJ (male, 8-20 wk old)[1]
Dosage: 50 µg; 100 µg; 300 µg
Administration: s.c.; administered twice at 7-day intervals
Result: Generated potent CTL responses that killed both unmanipulated C57BL/6 target cells and C3H.SW target cells coated with synthetic AAPDNRETF peptide.
Induced CTL activity comparable to that induced by priming with C57BL/6 cells.
Animal Model: C3H.SW/SnJ (male, 8-20 wk old, donor); C57BL/6J (male, 8-20 wk old, recipient, irradiated 950 rads)[1]
Dosage: 300 µg (donor priming)
Administration: s.c.; administered twice at 7-day intervals (donor priming)
Result: Caused 6/7 recipient mice to develop cutaneous GVHD (hair loss, erythematous lesions, skin ulcerations) starting around day 25, with lesions worsening up to day 60.
Induced significant thymic hypoplasia, with a 50% reduction in total Thy1.2+ thymocytes, CD4+CD8+ double positive thymocytes, CD4+TCRαβ high single positive thymocytes, and CD8+TCRαβ high single positive thymocytes compared to control recipients by day 60.
Maintained 100% recipient survival through day 60.
Molecular Weight

1020.05

Formula

C43H65N13O16
CAS No.
Sequence

Ala-Ala-Pro-Asp-Asn-Arg-Glu-Thr-Phe
Sequence Shortening

AAPDNRETF
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Purity & Documentation
References
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AAPDNRETF Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AAPDNRETF179953-98-1C57BL/6J miceH-2D?minor histocompatibility antigenC3H.SW Con A blast cellsgraft-versus-host diseasethymic hypoplasiaT2D^b cellsC57BL/6cytotoxic T lymphocytesC3H.SW/SnJ miceInhibitorinhibitorinhibit

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