Agar, meets USP testing specifications 

Agar, meets USP testing specifications is a high-quality selective growth support and substrate for non-adherent cells. Agar, meets USP testing specifications effectively supports the growth, colony formation and metachromatic matrix production of chondrocytes, and also facilitates the isolation and differentiation of pure chondrocyte strains by restricting the proliferation of fibroblast-like cells. Chondrocytes grown in Agar, meets USP testing specifications can be successfully transferred to a liquid suspension culture system, where they continue to proliferate while retaining the characteristics exhibited during growth in agar^[1][2].

Agar meeting USP testing specifications is used for clonal culture, phenotypic identification, purification and screening, and medium performance evaluation of chick embryo chondrocytes^[2].
1. Preparation of soft agar culture system
Unpurified Difco bacterial agar and tryptone phosphate are used to prepare the basal agar layer. Diluted single-cell suspension (10³ cells/60 mm culture dish) is inoculated on the upper layer. Modified Eagle's medium or F-12 medium containing 10% fetal bovine serum serves as the culture medium, and aseptic operation is performed throughout the process.
2. Results of clonal culture and phenotypic maintenance of chondrocytes
(1) After inoculation, chondrocytes derived from tibiae/femora of 13-day-old chick embryos initiate division and secrete chondromucoprotein matrix within several days, and form colonies containing more than 1000 cells in 3 weeks;
(2) Chondrocyte clones isolated from agar (designated as Ag series), after being passaged to monolayer culture, can still form colonies and produce metachromatic matrix when re-inoculated into soft agar, thus stably maintaining the differentiated phenotype of chondrocytes;
(3) Agarose can replace agar to achieve the same chondrocyte colony-forming effect.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9002-18-0

[Agar, meets USP testing specifications]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. tanley Borowicz. The Soft Agar Colony Formation Assay. Journal of Visualized Experiments. 10/27/2014.

  [2]. Horwitz A L, et al. The growth of cartilage cells in soft agar and liquid suspension[J]. The Journal of Cell Biology, 1970, 45(2): 434.