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HPV18 E6 Antibody

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HPV18 E6 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to HPV18 E6.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    IHC-P, IHC-F, ELISA, IF-Tissue

  • Reactivity :

    HPV18

  • Formulation:

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
  • HPV18 E6 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:100-500 1:100-500 1:100-500 1:5000-10000

Product Details

Description

HPV18 E6 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to HPV18 E6.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    HPV18
  • Observed Molecular Weight
    Observed band size: 14 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 12 kDa
Immunogen

KLH conjugated synthetic peptide derived from HPV18 E6 : 75-158/158

Purification

affinity purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    HPV18 E6 is the core oncoprotein encoded by high-risk human papillomavirus type 18, approximately 150-160 aa. It contains two zinc finger domains and a C-terminal PDZ binding motif. It has no enzymatic activity itself and exerts its carcinogenic effect by hijacking host proteins. Its core function is to form a heterodimer with the E3 ubiquitin ligase E6AP, recruiting and polyubiquitinizing p53, causing it to be degraded by the proteasome, thereby abolishing its "genome guardian" function. In addition, E6 synergistically inhibits p53 through multiple mechanisms, including binding to CBP/p300, inhibiting p53 phosphorylation, and cytoplasmic isolation; it degrades PDZ domain tumor suppressor proteins such as DLG1, SCRIB, and MAGI via C-terminal PBM, disrupting cell polarity and tight junctions; it independently activates hTERT/telomerase to achieve cell immortalization; it degrades Bak to inhibit apoptosis; and it binds to IRF3 to achieve immune escape. E6 is a key driver and important target of cervical cancer (especially HPV18-related cervical adenocarcinoma, such as HeLa cells) and other HPV-related malignancies.

[1]. Tang D, et al. HPV18 E6 inhibits α-ketoglutarate-induced pyroptosis of esophageal squamous cell carcinoma cells via the P53/MDH1/ROS/GSDMC pathway. FEBS Open Bio. 2023 Aug;13(8):1522-1535. [Content Brief]

HPV18 E6 Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • HPV18 E6 Antibody Product Image