Stopped-flow kinetic analysis using Hadamard transform time-of-flight mass spectrometry
- Anal Chem. 2010 Oct 15;82(20):8650-7. doi: 10.1021/ac101899n.
Affiliations
- 1. Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA.
PMID: 20843011
DOI: 10.1021/ac101899n
Abstract
A home-built stopped-flow apparatus is interfaced to a Hadamard transform time-of-flight mass spectrometer, which permits study of reaction kinetics with a time between reaction initiation and observation as short as about 100 ms and a sampling rate of chemical change that can approach 1 ms. This technique is applied to the trypsin-catalyzed hydrolysis of several peptides and is validated by comparing the results with literature values as well as to optical data obtained with the present stopped-flow apparatus. In addition, we report a kinetic study of the action of trypsin on a peptide having more than one cleavage site.