ATTO 633 

ATTO 633 is a fluorescent dye with an absorption peak at approximately 630 nm and a fluorescence emission peak at 651 nm. ATTO 633 can be used in nanomechanical photothermal microscopy studies^[1].

Guide (The following is our recommended protocol, which serves only as a guideline and should be modified according to your specific needs)^[1].
1. Stock Solution Preparation
1.1 Prepare a 1 mg/mL ATTO 633 solution as the stock solution.
2. Working Solution Preparation
2.1 Take 1 μL of ATTO 633 stock solution and dilute it with ultrapure deionized water at a ratio of 1:1000.
3. Experimental Procedures
3.1 Suspend gold nanoparticles with different diameters in 0.1 mM PBS, then dilute each with deionized water at a ratio of 1:40 at room temperature.
3.2 Filter the diluted solution through a polytetrafluoroethylene membrane syringe filter with a pore size of 200 nm to reduce aggregation.
3.3 Spin-coat the filtered solution onto silicon nitride rollers at rotational speeds of 2000 rpm and 4000 rpm for 20 s respectively to achieve uniform distribution of nanoparticles.
3.4 Pipette the ATTO 633 working solution onto the silicon nitride sample stage at room temperature, and measure immediately after air-drying at room temperature.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

588.18

C₃₅H₄₂ClN₃O₃

2982225-57-8

O=C(CCCN(C)C(C1=C(C2=C3C=C/C(C=C3C(C)(C)C4=CC5=C(CCCN5CC)C=C24)=[N+](C)/C)C=CC=C1)=O)O.[Cl-]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Chien MH, et al. Single-molecule optical absorption imaging by nanomechanical photothermal sensing. Proc Natl Acad Sci U S A. 2018 Oct 30;115(44):11150-11155.  [Content Brief]