L-Ascorbic acid 2-phosphate trisodium
Based on 10 publication(s) in Google Scholar
L-Ascorbic acid 2-phosphate trisodium (2-Phospho-L-ascorbic acid trisodium) is a long-acting vitamin C derivative that can stimulate collagen formation and expression. L-Ascorbic acid 2-phosphate trisodium (2-Phospho-L-ascorbic acid trisodium) can be used as a culture medium supplement for the osteogenic differentiation of human adipose stem cells (hASCs). L-Ascorbic acid 2-phosphate trisodium (2-Phospho-L-ascorbic acid trisodium) increases alkaline phosphatase (ALP) activity and expression of runx2A in hASCs during the osteogenic differentiation.
For research use only. We do not sell to patients.
- Purity: 98.60%
- CAS No.: 66170-10-3
- Formula: C6H6Na3O9P
- Molecular Weight:322.05
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) L-Ascorbic acid 2-phosphate trisodium
More- Adv Mater. 2025 Jul 14:e2501306. [Abstract]
- Autophagy. 2023 Feb;19(2):632-643. [Abstract]
- Adv Sci (Weinh). 2025 Nov 21:e08472. [Abstract]
- Adv Sci (Weinh). 2024 Dec;11(46):e2400502. [Abstract]
- Food Hydrocoll. 2026 Mar.
- Int J Biol Macromol. 2025 Dec 12;338(Pt 1):149672. [Abstract]
- Biomolecules. 2025 Nov 19;15(11):1623. [Abstract]
- J Biomed Mater Res A. 2026 Feb;114(2):e70031. [Abstract]
- Cell Transplant. 2025 Jan-Dec:34:9636897251376125. [Abstract]
- Eng Life Sci. 2025 Oct 13;25(10):e70050. [Abstract]
All Endogenous Metabolite Isoforms
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Biological Activity
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Human Endogenous Metabolite |
L-Ascorbic acid 2-phosphate (0.1-1.5 mM; 2 to 3 weeks with medium exchange every 2 to 3 days) trisodium significantly stimulates cell growth, whereas addition of l-Ascorbic acid (Asc) achieves only weak growth stimulation. A combination of Asc-2P and bFGF significantly increases cell growth, but supplementation with EGF and/or insulin does not have any additional effect [1]. L-Ascorbic acid 2-phosphate (50 μM-250 μM) trisodium is needed for the effective osteogenic differentiation of human adipose stem cells (hASCs), and higher concentrations of AsA2-P results in increased runx2 expression and ALP activity. The highest proliferation, ALP activity and runx2 expression is achieved with 150 μM AsA2-P and 10 nM dexamethasone (Dex), and 250 μM AsA2-P and 5 nM Dex[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 66170-10-3
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Appearance Solid
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Molecular Weight 322.05
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Formula C6H6Na3O9P
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Color White to off-white
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SMILES
O[C@@H](CO)[C@@H](O1)C(O[Na])=C(OP(O[Na])(O[Na])=O)C1=O
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Synonyms
2-Phospho-L-ascorbic acid trisodium
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (10)
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Journal Impact Factor
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Most Recent
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Adv Mater
Piezoelectric Injectable Anti-Adhesive Hydrogel to Promote Endogenous Healing of Tendon Injuries. [Abstract]2025 Jul 14:e2501306. PMID: 40658817 -
Autophagy
Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis. [Abstract]2023 Feb;19(2):632-643. PMID: 35786294 -
Adv Sci (Weinh)
Targeted Inhibition of CD74+ Macrophages by Luteolin via CEBPB/P65 Signaling Ameliorates Osteoarthritis Progression. [Abstract]2025 Nov 21:e08472. PMID: 41268703 -
Adv Sci (Weinh)
Insufficient Mechanical Loading Downregulates Piezo1 in Chondrocytes and Impairs Fracture Healing Through ApoE-Induced Senescence. [Abstract]2024 Dec;11(46):e2400502. PMID: 39418070 -
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Int J Biol Macromol
Downregulation of the biological macromolecule Spondin-2 disrupts maternal-fetal immune tolerance and trophoblast invasion, driving unexplained recurrent pregnancy loss. [Abstract]2025 Dec 12;338(Pt 1):149672. PMID: 41391791 -
Biomolecules
Mitochondrial Imaging and Transcriptome Analysis of Bone Mesenchymal Stem Cells During Osteogenesis Under Different Culture Conditions. [Abstract]2025 Nov 19;15(11):1623. PMID: 41301543 -
J Biomed Mater Res A
Injectable Butyl Glycidyl Ether-Modified Methylcellulose Thermosensitive Hydrogel for Topical Delivery of Vancomycin Hydrochloride in the Treatment of Osteomyelitis. [Abstract]2026 Feb;114(2):e70031. PMID: 41586469 -
Cell Transplant
Adipose-derived stem cells inhibit dendritic cell migration by secreting tumor necrosis factor-α-stimulated gene 6 to improve the allogeneic skin transplantation survival rate in mice. [Abstract]2025 Jan-Dec:34:9636897251376125. PMID: 41108126 -
Eng Life Sci
Comparative Study of Porcine Mesenchymal Stem Cells Behavior and Lipid Metabolism on Plant-Based Scaffolds and Two-Dimensional Systems for Cultivated Fat. [Abstract]2025 Oct 13;25(10):e70050. PMID: 41098339
Solvent & Solubility
H2O : 150 mg/mL (465.77 mM; Need ultrasonic)
DMSO : 6 mg/mL (18.63 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 120 mg/mL (372.61 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Shima N, et al. Increased proliferation and replicative lifespan of isolated human corneal endothelial cells with L-ascorbic acid 2-phosphate.Invest Ophthalmol Vis Sci. 2011 Nov 7;52(12):8711-7. [Content Brief]
[2]. Kurata S, et al. Epidermal growth factor inhibits transcription of type I collagen genes and production of type I collagen in cultured human skin fibroblasts in the presence and absence of L-ascorbic acid 2-phosphate, a long-acting vitamin C derivative.J Biol Chem. 1991 May 25;266(15):9997-10003. [Content Brief]
[3]. Kyllönen L, et al. Effects of different serum conditions on osteogenic differentiation of human adipose stem cells in vitro.Stem Cell Res Ther. 2013 Feb 15;4(1):17. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 3.1051 mL | 15.5255 mL | 31.0511 mL | 77.6277 mL |
| 5 mM | 0.6210 mL | 3.1051 mL | 6.2102 mL | 15.5255 mL | |
| 10 mM | 0.3105 mL | 1.5526 mL | 3.1051 mL | 7.7628 mL | |
| 15 mM | 0.2070 mL | 1.0350 mL | 2.0701 mL | 5.1752 mL | |
| H2O | 20 mM | 0.1553 mL | 0.7763 mL | 1.5526 mL | 3.8814 mL |
| 25 mM | 0.1242 mL | 0.6210 mL | 1.2420 mL | 3.1051 mL | |
| 30 mM | 0.1035 mL | 0.5175 mL | 1.0350 mL | 2.5876 mL | |
| 40 mM | 0.0776 mL | 0.3881 mL | 0.7763 mL | 1.9407 mL | |
| 50 mM | 0.0621 mL | 0.3105 mL | 0.6210 mL | 1.5526 mL | |
| 60 mM | 0.0518 mL | 0.2588 mL | 0.5175 mL | 1.2938 mL | |
| 80 mM | 0.0388 mL | 0.1941 mL | 0.3881 mL | 0.9703 mL | |
| 100 mM | 0.0311 mL | 0.1553 mL | 0.3105 mL | 0.7763 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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