Cyanine5 alkyne
Based on 1 Customer Validation
Cyanine5 alkyne (Alkyne-Cy5) is a fluorescent dye used to label azide proteins and can be used to analyse post-translational modifications of proteins, glycosylation etc. Cyanine5 alkyne can also be used as a mitochondrial OXPHOS inhibitor to inhibit the growth of cancer stem cells (CSC). Cyanine5 alkyne is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
For research use only. We do not sell to patients.
- Purity: 98.15%
- CAS No.: 1223357-57-0
- Formula: C35H42ClN3O
- Molecular Weight:556.18
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Cyanine5 alkyne (Alkyne-Cy5) (1-1000 nM) significantly inhibits 3D mammosphere formation in MCF7 cells between 500 nM and 1000 nM and can act as a mitochondrial oxidative phosphorylation (OXPHOS) inhibitor, inducing glycolysis to compensate for mitochondrial ATP depletion[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 1223357-57-0
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Appearance Solid
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Molecular Weight 556.18
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Formula C35H42ClN3O
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Color Brown to dark brown
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SMILES
C#CCNC(CCCCC[N+]1=C(/C=C/C=C/C=C2N(C)C3=C(C=CC=C3)C\2(C)C)C(C)(C)C4=C1C=CC=C4)=O.[Cl-]
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Synonyms
Alkyne-Cy5
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 125 mg/mL (224.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Amanda R Burnham-Marusich, et al. Size-matched alkyne-conjugated cyanine fluorophores to identify differences in protein glycosylation. Electrophoresis. 2014 Sep;35(18):2621-5. [Content Brief]
[2]. Camillo Sargiacomo, et al. MitoTracker Deep Red (MTDR) Is a Metabolic Inhibitor for Targeting Mitochondria and Eradicating Cancer Stem Cells (CSCs), With Anti-Tumor and Anti-Metastatic Activity In Vivo. Front Oncol. 2021 Jul 30;11:678343. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7980 mL | 8.9899 mL | 17.9798 mL | 44.9495 mL |
| 5 mM | 0.3596 mL | 1.7980 mL | 3.5960 mL | 8.9899 mL | |
| 10 mM | 0.1798 mL | 0.8990 mL | 1.7980 mL | 4.4949 mL | |
| 15 mM | 0.1199 mL | 0.5993 mL | 1.1987 mL | 2.9966 mL | |
| 20 mM | 0.0899 mL | 0.4495 mL | 0.8990 mL | 2.2475 mL | |
| 25 mM | 0.0719 mL | 0.3596 mL | 0.7192 mL | 1.7980 mL | |
| 30 mM | 0.0599 mL | 0.2997 mL | 0.5993 mL | 1.4983 mL | |
| 40 mM | 0.0449 mL | 0.2247 mL | 0.4495 mL | 1.1237 mL | |
| 50 mM | 0.0360 mL | 0.1798 mL | 0.3596 mL | 0.8990 mL | |
| 60 mM | 0.0300 mL | 0.1498 mL | 0.2997 mL | 0.7492 mL | |
| 80 mM | 0.0225 mL | 0.1124 mL | 0.2247 mL | 0.5619 mL | |
| 100 mM | 0.0180 mL | 0.0899 mL | 0.1798 mL | 0.4495 mL |
Cyanine5 alkyne Related Classifications
HY-137042 Related Classifications
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