Cat. No.: HY-123834

FEN1-IN-1

8 Cited Publications

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Based on 8 publication(s) in Google Scholar

FEN1-IN-1 (compound 1) is a small molecule flap endonuclease 1 (FEN1) inhibitor with antitumor activity. FEN1-IN-1 binds to the active site of FEN1 and partly achieves inhibition by the co-ordination of Mg²⁺ ions. FEN1-IN-1 initiaties a DNA damage response and activates the ATM checkpoint signalling pathway, the phosphorylation of histone H2AX and the ubiquitination of FANCD2 in mammalian cells. FEN1-IN-1 is promising for research of cancers.

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Pureté: 99.85%
CAS No.: 824983-91-7
Formule: C₁₅H₁₂N₂O₅S
Masse moléculaire:332.33

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

FEN1-IN-1 Chemical Structure

CAS No.: 824983-91-7

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Activité biologique
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (8)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Bioz

Activité biologique

Description

IC₅₀ & Target

FEN1^[1]

Voir tous les produits spécifiques à Isoform ATM/ATR :

In Vitro

FEN1-IN-1 (0-30 μM, 3 days) inhibits 212 cell-lines growth with a mean GI 50 of 15.5 μM^[1].
FEN1-IN-1 (0.2-20 μM, 24 h) induces Hela cells disrupted for MRE11A more sensitive and decreasea the survival of the MRE11A deficient cell-lines due to cytotoxicity^[1].
FEN1-IN-1 (0-10 μM, 24 h) inhibits both FEN1 and EXO1 function and induces a DNA damage response to activate the signalling pathways in SW620 and HCT-116 cells^[1].
FEN1-IN-1 (10 μM, 24 h) leads to replication fork instabilities that, if left unrepaired, can lead to cell death^[1].

Tissue-specific sensitivity to FEN1-IN-1 (GI₅₀: μM)^[1]

FEN1-IN-1	Bladder	Breast	Colorectal	Gastric	Liver	Lung	Pancreatic	Prostate	Gynae	Head & Neck
Cell-lines screened	19	22	21	25	21	37-	24	7	21	15

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 824983-91-7
Appearance Solid
Masse moléculaire 332.33
Formule C₁₅H₁₂N₂O₅S
Color White to off-white
SMILES

O=C1N(CC2OC3=CC=CC=C3OC2)C(C=CS4)=C4C(N1O)=O
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (8)

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Journal Impact Factor

Chem Eng J

2023 Aug 15, 470, 144407.
Biosens Bioelectron

Homogeneous electrochemical protein assay based on repeated DNA hairpin self-folding and catalyzed extension. [Abstract]

2025 Oct 25:293:118160. PMID: 41151473
Adv Healthc Mater

Ultrasensitive Detection of FEN1 Activity for Cancer Diagnosis Using a CRISPR/Cas13a-Based Triple Cascade Amplification System. [Abstract]

2025 Mar;14(6):e2404411. PMID: 40029034
ACS Appl Mater Interfaces

Self-Powered FEN1 Biosensor Based on Accelerated CRISPR/Cas Trans-Cleavage around Porous Fe3O4 Nanoparticles. [Abstract]

2024 Oct 16;16(41):55181-55188. PMID: 39361498
Sens Actuators B Chem

2023 Oct 15, 393, 134265.
Anal Chem

Signal-Amplified Detection of the Tumor Biomarker FEN1 Based on Cleavage-Induced Ligation of a Dumbbell DNA Probe and Rolling Circle Amplification. [Abstract]

2021 Feb 16;93(6):3287-3294. PMID: 33529005
ChemElectroChem

2023 Mar 16.
University of Alberta

2025.

Solvant et solubilité

In Vitro:

DMSO : 250 mg/mL (752.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.0091 mL

15.0453 mL

30.0906 mL

5 mM

0.6018 mL

3.0091 mL

6.0181 mL

10 mM

0.3009 mL

1.5045 mL

3.0091 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (6.26 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (6.26 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (6.26 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (277 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Ward TA, et al. Small molecule inhibitors uncover synthetic genetic interactions of human flap endonuclease 1 (FEN1) with DNA damage response genes. PLoS One. 2017 Jun 19;12(6):e0179278. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.0091 mL	15.0453 mL	30.0906 mL	75.2264 mL
	5 mM	0.6018 mL	3.0091 mL	6.0181 mL	15.0453 mL
	10 mM	0.3009 mL	1.5045 mL	3.0091 mL	7.5226 mL
	15 mM	0.2006 mL	1.0030 mL	2.0060 mL	5.0151 mL
	20 mM	0.1505 mL	0.7523 mL	1.5045 mL	3.7613 mL
	25 mM	0.1204 mL	0.6018 mL	1.2036 mL	3.0091 mL
	30 mM	0.1003 mL	0.5015 mL	1.0030 mL	2.5075 mL
	40 mM	0.0752 mL	0.3761 mL	0.7523 mL	1.8807 mL
	50 mM	0.0602 mL	0.3009 mL	0.6018 mL	1.5045 mL
	60 mM	0.0502 mL	0.2508 mL	0.5015 mL	1.2538 mL
	80 mM	0.0376 mL	0.1881 mL	0.3761 mL	0.9403 mL
	100 mM	0.0301 mL	0.1505 mL	0.3009 mL	0.7523 mL

Keywords

FEN1-IN-1

8 Cited Publications

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FEN1-IN-1

Cell Cycle/DNA Damage Compound Library

Immunology/Inflammation Compound Library

PI3K/Akt/mTOR Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Oxygen Sensing Compound Library

Glycolysis Compound Library

Cytoskeleton Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Glucose Metabolism Compound Library

Targeted Diversity Library

Heterocyclic Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

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Protein Kinase Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) FEN1-IN-1

Voir tous les produits spécifiques à Isoform ATM/ATR

Activité biologique

Chemical Information

Publications (8)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

FEN1-IN-1

Cell Cycle/DNA Damage Compound Library

Immunology/Inflammation Compound Library

PI3K/Akt/mTOR Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Oxygen Sensing Compound Library

Glycolysis Compound Library

Cytoskeleton Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Glucose Metabolism Compound Library

Targeted Diversity Library

Heterocyclic Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Protein Kinase Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) FEN1-IN-1

Voir tous les produits spécifiques à Isoform ATM/ATR

Activité biologique

Chemical Information

Publications (8)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

FEN1-IN-1 Related Classifications

Cat. No.: HY-123834