WEN05-03
WEN05-03 is a EscN ATPase inhibitor. WEN05-03 blocks the active site of EscN ATPase and competitively inhibits its ATP hydrolysis activity. WEN05-03 completely blocks actin cluster formation, reduces actin pedestal formation, and decreases the toxicity of infected mammalian cells. WEN05-03 can be used in studies related to enteropathogenic E. coli (EPEC) infection.
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- CAS No.: 2419180-34-8
- Formule: C17H14N4O4S
- Masse moléculaire:370.38
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
WEN05-03 acts as a potent competitive inhibitor of purified recombinant enteropathogenic E. coli EscN ATPase, with a Ki value of 16 μM[1].
WEN05-03 (100 μM; 24 h) exhibits extremely low toxicity to HeLa and BALB/3T3 cells, with only 7% growth inhibition observed in BALB/3T3 cells at the concentration of 100 μM, while no significant toxicity is detected in HeLa cells[1].
WEN05-03 (100 μM; 2 h) completely blocks the formation of actin clusters in HeLa cells infected with enteropathogenic E. coli (EPEC), with levels comparable to those in uninfected mock-treated cells[1].
WEN05-03 competitively inhibits the ATP hydrolysis activity of the EPEC EscN type III secretion system (T3SS) ATPase, reduces the toxicity of EPEC to infected HeLa cells, and exerts no effect on bacterial growth or host cell viability[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HeLa cells (human epithelial cell line), BALB/3T3 cells (mouse fibroblast cell line)
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Concentration:100 μM
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Incubation Time:24 h
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Result:Showed no discernible effect on cell viability, minimal interference with mitochondrial potential, and only low levels of apoptosis induction in HeLa cells.
Caused only 7% growth inhibition in BALB/3T3 cells as measured by MTT assay.
Chemical Information
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CAS No. 2419180-34-8
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Masse moléculaire 370.38
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Formule C17H14N4O4S
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SMILES
O=C(N)CSC=1C=CC=CC1C(=O)NC2=CC=C3NC(=O)C(=O)NC3=C2
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
[1]. Bzdzion L, et al. Design of small molecule inhibitors of type III secretion system ATPase EscN from enteropathogenic Escherichia coli. Acta Biochim Pol. 2017;64(1):49-63. [Content Brief]
[2]. Fasciano AC, et al. Promises and Challenges of the Type Three Secretion System Injectisome as an Antivirulence Target. EcoSal Plus. 2019 Feb;8(2):10.1128/ecosalplus.ESP-0032-2018. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
WEN05-03 Related Classifications
HY-147127 Related Classifications
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