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Art. -Nr.: HY-183704

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ISR activator-5

ISR activator-5 is an eIF2B modulator and integrated stress response (ISR) activator. ISR activator-5 stabilises the inactive I-state of eIF2B, inhibits protein synthesis, and activates canonical ISR pathways without inducing eIF2α phosphorylation. ISR activator-5 can be used for the research of cancer, neurologic and metabolic disorders.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Formel: C₅₂H₆₀F₆N₄O₆
Molecular Weight:951.05

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

ISR activator-5 Chemical Structure

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target^[1]

eIF2-β

In Vitro

ISR activator-5 (Compound B) (20 μM; 16 h) activates the CHOP::Luciferase ISR reporter in CHO-K1 cells with an EC₅₀ of 13 μM^[1].
ISR activator-5 (20 μM; 60 min) inhibits protein synthesis in CHO-K1 cells with an IC₅₀ of 11.74 μM^[1].
ISR activator-5 (30 μM; 1 h) inhibits translation initiation in HEK cells^[1].
ISR activator-5 (6 h) activates a canonical, PERK-specific ISR gene expression program in CHO cells, with significant induction of pathways linked to ISR-mediated stress adaptation^[1].
ISR activator-5 (20 μM; 1-24 h) induces endogenous ISR marker proteins (GADD34, ATF4, CHOP) in CHO parental cells starting at 1 hour, with peak induction at 6 hours, and this activity requires eIF2α phosphorylation at Ser51^[1].
ISR activator-5 (20 μM; 18 h) activates the CHOP::GFP ISR reporter in CHO cells with an EC₅₀ of 15 μM, and this activation is partially attenuated by co-treatment with 1 μM ISRIB^[1].
ISR activator-5 (escalating concentrations; 18 h) activates the CHOP::GFP ISR reporter in EIF2B4^L180F mutant CHO cells with an EC₅₀ of 22.63 μM, showing modestly reduced potency compared to wildtype CHO cells, and ISRIB cannot attenuate this activity^[1].
ISR activator-5 does not significantly activate the IRE1/XBP1 axis in CHO cells, demonstrating specificity for the ISR pathway^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

CHO parental cells
Concentration:

20 μM
Incubation Time:

1 h; 3 h; 6 h; 9 h; 12 h; 24 h
Result:

Induced expression of GADD34, ATF4, and CHOP beginning at 1 hour, with induction peaking at 6 hours, similar to thapsigargin-treated cells.
Showed no induction of these markers in eIF2α-S51A mutant cells treated with Compound B.

Chemical Information

Molecular Weight 951.05
Formel C₅₂H₆₀F₆N₄O₆
SMILES

O=C(C)N(C)CC1=CC=CC(C2=CC3=C(C(C(F)(F)F)=C2)CCCC3N(C)C(CCOCCOCCC(N(C)C4C(C=C(C5=CC=CC(CN(C)C(C)=O)=C5)C=C6C(F)(F)F)=C6CCC4)=O)=O)=C1
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Handling Instructions (2659 KB)

Verweise

[1]. Shilliday F, et al. A molecular stabiliser of an inhibitory eIF2B-eIF2(αP) complex activates the Integrated Stress Response. Nat Commun. Published online May 6, 2026. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

MOQ

Minimum order quantity

100 mg

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