Monoamine oxidase, plasma

Monoamine oxidase has some isoenzymes, the most typical of which are monoamine oxidase (MAO) A and MAOB. MAOA is usually inhibited by low concentrations of Clorgyline (HY-14197A) and MAOB is usually inhibited by low concentrations of l-deprenyl^[3].
Monoamine Oxidase Assay
1. Solution Preparation
1) Substrate: 1.0% v/v (0.10 M) benzylamine in 67 mM potassium phosphate buffer, pH 7.2.
2) Enzyme solution: Dissolve to a concentration of 10 mg/mL in 67 mM potassium phosphate buffer (pH 7.2) and store at 2-8°C for up to 1 week.
2. Assay Procedure
1) Adjust spectrophotometer to 250 nm and 25°C.
2) Pipette 2.8 mL 67 mM Potassium phosphate buffer pH 7.2 and 0.1 mL substrate solution into each cuvette.
3) Incubate in spectrophotometer for 3-4 minutes to reach temperature equilibrium.
4) Add 0.1 mL enzyme solution and record the increase in A250 over 6-8 minutes. Calculate ΔA250/min from the linear portion of the curve. A 2-3 minute delay may occur.
5) Calculation
Units/g = (ΔA250/min x 1000)/(13.0 x mg enzyme/mL reaction mixture)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.