Simultaneous measurement of nicotinamide and its catabolites, nicotinamide N-oxide, N(1)-methyl-2-pyridone-5-carboxamide, and N(1)-methyl-4-pyridone-3-carboxamide, in mice urine

  • Biosci Biotechnol Biochem. 2014;78(8):1306-9. doi: 10.1080/09168451.2014.918495.
Akihiro Maeta  1 ,  Mitsue Sano ,  Tsutomu Fukuwatari ,  Katsumi Shibata
Affiliations
  • 1. a Department of Nutrition , School of Human Cultures, The University of Shiga Prefecture , Hikone , Japan.
PMID: 25130730 DOI: 10.1080/09168451.2014.918495
Abstract

Nicotinamide N-oxide is a major nicotinamide catabolite in mice but not in humans and rats. A high-performance liquid chromatographic method for the simultaneous measurement of nicotinamide, nicotinamide N-oxide, N(1)-methyl-2-pyridone-5-carboxamide, and N(1)-methyl-4-pyridone-3-carboxamide in mice urine was developed by modifying the mobile phase of a reported method for measurement of nicotinamide N-oxide.

Keywords
HPLC; mouse; nicotinamide N-oxide; urine; vitamin.
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