An activatable fluorescent probe for imaging endogenous nitric oxide via the eNOS enzymatic pathway

  • Bioorg Med Chem Lett. 2022 Mar 1;59:128544. doi: 10.1016/j.bmcl.2022.128544.
Zhirong Ouyang  1 ,  Muye Ma  2 ,  Kai Yin  1 ,  Nan Guo  1 ,  Wei Fu  1 ,  Wei Guo  3 ,  Xianfeng Gu  4
Affiliations
  • 1. School of Pharmacy & Minhang Hospital, Fudan University, Shanghai 201301, China.
  • 2. Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai 201301, China.
  • 3. Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai 201301, China. Electronic address: [email protected].
  • 4. School of Pharmacy & Minhang Hospital, Fudan University, Shanghai 201301, China. Electronic address: [email protected].
PMID: 35031453 DOI: 10.1016/j.bmcl.2022.128544
Abstract

Nitric oxide (NO) is an essential cellular messenger molecule involved in various physiological and pathological processes. Thus, monitoring the dynamic presence of endogenous NO in living cells is of great significance. In this paper, we developed an activatable fluorescent nanoprobe BOD-NH-NP for endogenous NO detection. In the probe BOD-NH-NP, the fast responding reaction site towards NO, incorporating a BODIPY Fluorescent Dye with good optical features, enables the probe to be applied for the detection of endogenous NO via the eNOS enzymatic pathway in living cells and screening nitric oxide synthases (NOSs) inhibitors and agonists.

Keywords
Fluorescent probe; Nitric oxide; eNOS.
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