Anti-Inflammatory Effects of Tegoprazan in Lipopolysaccharide-Stimulated Bone-Marrow-Derived Macrophages

  • Int J Mol Sci. 2023 Sep 26;24(19):14589. doi: 10.3390/ijms241914589.
Gong-Ho Han  1  2 ,  Seong-Jun Kim  1  2 ,  Wan-Kyu Ko  1  2 ,  Je-Beom Hong  3 ,  Seung-Hun Sheen  1 ,  Min-Jai Cho  4 ,  Seil Sohn  1
Affiliations
  • 1. Department of Neurosurgery, CHA Bundang Medical Center, CHA University, 59 Yatap-ro, Bundang-gu, Seongnam-si 13496, Gyeonggi-do, Republic of Korea.
  • 2. Department of Life Science, CHA University, Boondagger, Seongnam-si 13493, Gyeonggi-do, Republic of Korea.
  • 3. Department of Neurosurgery, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul 16419, Republic of Korea.
  • 4. Department of Neurosurgery, Chungbuk National University College of Medicine, Chungbuk National University Hospital, Seowon-gu, Cheongju-si 28644, Chungcheong-do, Republic of Korea.
PMID: 37834036 DOI: 10.3390/ijms241914589
Abstract

The purpose of this study was to investigate the anti-inflammatory effect of tegoprazan (TEGO) in lipopolysaccharide (LPS)-stimulated bone-marrow-derived macrophages (BMMs). To this end, compared to methylprednisolone (MP; positive control), we evaluated whether TEGO effectively differentiates LPS-stimulated BMMs into M2-phenotype macrophages. Moreover, the expression of pro- and anti-inflammatory cytokines genes influenced by TEGO was measured using quantitative real-time polymerase chain reaction (qRT-PCR) analysis. TEGO was found to reduce nitric oxide (NO) production in BMMs significantly. In addition, TEGO significantly decreased and increased the gene expression levels of pro-inflammatory and anti-inflammatory cytokines, respectively. In addition, we evaluated the phosphorylated values of the extracellular signal-regulatory kinase (ERK) and p38 in the mitogen-activated protein (MAP) kinase signaling pathway through Western blotting. TEGO significantly reduced the phosphorylated values of the ERK and p38. In Other words, TEGO suppressed the various pro-inflammatory responses in LPS-induced BMMs. These results show that TEGO has the potential to be used as an anti-inflammatory agent.

Keywords
LPS-induced inflammation; anti-inflammation substance; bone-marrow-derived macrophages; inflammation; tegoprazan.
Products