PRKAG1 - protein kinase AMP-activated non-catalytic subunit gamma 1 Gene

Also Known as AMPKG

Species: Homo sapiens

Gene Type: protein coding
Gene ID: 5571

About PRKAG1

Cytogenetic location: 12q13.12 Genomic coordinates (GRCh38): 12:49,002,274-49,018,776 (from NCBI)

This gene has 20 transcripts (splice variants), 216 orthologues and 2 paralogues. Ubiquitous expression in heart (RPKM 18.1), adrenal (RPKM 16.8) and 25 other tissues.

Summary

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates Acetyl-CoA Carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key Enzymes involved in regulating de novo biosynthesis of fatty acid and Cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

PRKAG1 Products (3)

mRNA Protein Name
NM_001206709.2 NP_001193638.1 5'-AMP-activated protein kinase subunit gamma-1 isoform 3
NM_001206710.2 NP_001193639.1 5'-AMP-activated protein kinase subunit gamma-1 isoform 4
NM_002733.5 NP_002724.1 5'-AMP-activated protein kinase subunit gamma-1 isoform 1

Gene Ontology

  • Molecular Function
  • Biological Process
  • Cellular Component
Molecular Function GO Annotation Evidence Références Source
enables protein binding IPI
IPI: Inferred from physical interaction
16306228 GOA
enables protein kinase activity IDA
IDA: Inferred from direct assay
17028174 GOA
enables protein kinase binding IDA
IDA: Inferred from direct assay
10698692 GOA
enables protein kinase regulator activity IDA
IDA: Inferred from direct assay
24563466 GOA
Biological Process GO Annotation Evidence Références Source
involved in cellular response to nutrient levels IDA
IDA: Inferred from direct assay
25412657 GOA
involved in positive regulation of gene expression IDA
IDA: Inferred from direct assay
17028174 GOA
involved in protein phosphorylation IDA
IDA: Inferred from direct assay
17028174 GOA
Cellular Component GO Annotation Evidence Références Source
part of nucleotide-activated protein kinase complex IPI
IPI: Inferred from physical interaction
9693118 GOA
located in nucleus IDA
IDA: Inferred from direct assay
9693118 GOA
EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

PRKAG1 Protein Structure

CBS

CBS: CBS domain (45 - 96)

CBS

CBS: CBS domain (128 - 177)

CBS

CBS: CBS domain (191 - 248)

CBS

CBS: CBS domain (276 - 323)

  • 0
  • 100
  • 200
  • 300
  • 331 a.a.
Protein Preferred Names Protein Names

5'-AMP-activated protein kinase subunit gamma-1

  • 5'-AMP-activated protein kinase, gamma-1 subunit

PRKAG1 Protein-protein interaction Information

Type
Protein Name Protein ID Interactor Interactor Species Interactor ID Detection Method Références
Intra
PRKAG1 P54619 KRTAP10-5 Homo sapiens P60370
Validated Y2H
32296183
Intra
PRKAG1 P54619 KRTAP4-2 Homo sapiens Q9BYR5
Validated Y2H
25416956
Intra
PRKAG1 P54619 KRTAP4-2 Homo sapiens Q9BYR5
Y2H Array
25416956
Intra
PRKAG1 P54619 TEKT1 Homo sapiens Q969V4
Validated Y2H
25416956
Intra
PRKAG1 P54619 TEKT1 Homo sapiens Q969V4
Validated Y2H
32296183
Intra
PRKAG1 P54619 TEKT1 Homo sapiens Q969V4
Y2H Prey Pooling
32296183
Intra
PRKAG1 P54619 TEKT1 Homo sapiens Q969V4
Y2H Array
32296183
Intra
PRKAG1 P54619 TEKT1 Homo sapiens Q969V4
Y2H Prey Pooling
25416956
Intra
PRKAG1 P54619 KRTAP10-11 Homo sapiens P60412
Validated Y2H
32296183
Intra
PRKAG1 P54619 KRTAP9-2 Homo sapiens Q9BYQ4
Validated Y2H
32296183
Intra
PRKAG1 P54619 PRKAB2 Homo sapiens O43741
Anti Tag CoIP
33961781
Intra
PRKAG1 P54619 PRKAB2 Homo sapiens O43741
Anti Tag CoIP
28514442
Intra
PRKAG1 P54619 PRKAB2 Homo sapiens O43741
Y2H Array
25416956
Intra
PRKAG1 P54619 PRKAB2 Homo sapiens O43741
Y2H Array
32296183
Intra
PRKAG1 P54619 PRKAB2 Homo sapiens O43741
Y2H Prey Pooling
32296183
Intra
PRKAG1 P54619 PRKAB2 Homo sapiens O43741
Anti Tag CoIP
20562859
Intra
PRKAG1 P54619 PRKAB2 Homo sapiens O43741
Y2H
21988832
Intra
PRKAG1 P54619 PRKAA1 Homo sapiens Q13131
Anti Bait CoIP
22363791
Intra
PRKAG1 P54619 PRKAA1 Homo sapiens Q13131
Anti Tag CoIP
33961781
Intra
PRKAG1 P54619 PRKAA1 Homo sapiens Q13131
Y2H Prey Pooling
25416956
Intra
PRKAG1 P54619 PRKAA1 Homo sapiens Q13131
Y2H Array
25416956
Intra
PRKAG1 P54619 PRKAA1 Homo sapiens Q13131
Anti Tag CoIP
20562859
Intra
PRKAG1 P54619 KRTAP9-8 Homo sapiens Q9BYQ0
Validated Y2H
32296183
Intra
PRKAG1 P54619 KRTAP1-1 Homo sapiens Q07627
Validated Y2H
32296183
Intra
PRKAG1 P54619 KRTAP1-1 Homo sapiens Q07627
Y2H Prey Pooling
32296183
Intra
PRKAG1 P54619 KRTAP1-1 Homo sapiens Q07627
Y2H Array
32296183
Intra
PRKAG1 P54619 PRKAA2 Homo sapiens P54646
Anti Tag CoIP
33961781
Intra
PRKAG1 P54619 PRKAA2 Homo sapiens P54646
Anti Tag CoIP
28514442
Intra
PRKAG1 P54619 PRKAA2 Homo sapiens P54646
Y2H Prey Pooling
25416956
Intra
PRKAG1 P54619 PRKAA2 Homo sapiens P54646
Y2H Prey Pooling
32296183
Intra
PRKAG1 P54619 PRKAA2 Homo sapiens P54646
Validated Y2H
32296183
Intra
PRKAG1 P54619 PRKAA2 Homo sapiens P54646
Y2H Array
25416956
Intra
PRKAG1 P54619 PRKAA2 Homo sapiens P54646
Anti Tag CoIP
20562859
Intra
PRKAG1 P54619 PRKAA2 Homo sapiens P54646
Y2H Array
32296183
Intra
PRKAG1 P54619 MEOX2 Homo sapiens Q6FHY5
Validated Y2H
32296183
Intra
PRKAG1 P54619 MEOX2 Homo sapiens Q6FHY5
Y2H Prey Pooling
32296183
Intra
PRKAG1 P54619 MEOX2 Homo sapiens Q6FHY5
Y2H Array
32296183
Intra
PRKAG1 P54619 TNFAIP1 Homo sapiens Q13829
Y2H Array
32296183
Intra
PRKAG1 P54619 TNFAIP1 Homo sapiens Q13829
Y2H Prey Pooling
32296183
Intra
PRKAG1 P54619 TNFAIP1 Homo sapiens Q13829
Validated Y2H
32296183
Intra
PRKAG1 P54619 MEOX1 Homo sapiens P50221
Y2H Prey Pooling
32296183
Intra
PRKAG1 P54619 MEOX1 Homo sapiens P50221
Y2H Array
32296183
Intra
PRKAG1 P54619 PRKAB1 Homo sapiens Q9Y478
Anti Tag CoIP
18480843
Intra
PRKAG1 P54619 PRKAB1 Homo sapiens Q9Y478
Anti Tag CoIP
28514442
Intra
PRKAG1 P54619 PRKAB1 Homo sapiens Q9Y478
Anti Bait CoIP
22363791
Intra
PRKAG1 P54619 PRKAB1 Homo sapiens Q9Y478
Validated Y2H
32296183
Intra
PRKAG1 P54619 PRKAB1 Homo sapiens Q9Y478
Y2H Array
32296183
Intra
PRKAG1 P54619 PRKAB1 Homo sapiens Q9Y478
Y2H Prey Pooling
32296183
Intra
PRKAG1 P54619 PRKAB1 Homo sapiens Q9Y478
Anti Tag CoIP
20562859
Intra
PRKAG1 P54619 PRKAB1 Homo sapiens Q9Y478
Anti Tag CoIP
33961781
Intra
PRKAG1 P54619 KRTAP4-12 Homo sapiens Q9BQ66
Validated Y2H
32296183
Intra
PRKAG1 P54619 CBS Homo sapiens P35520
Y2H Prey Pooling
25416956
Intra
PRKAG1 P54619 PLEKHF2 Homo sapiens Q9H8W4
Y2H Array
32296183
Intra
PRKAG1 P54619 PLEKHF2 Homo sapiens Q9H8W4
Y2H Prey Pooling
32296183
Intra
PRKAG1 P54619 PLEKHF2 Homo sapiens Q9H8W4
Validated Y2H
32296183
Intra
PRKAG1 P54619 PAX6 Homo sapiens P26367
Y2H Prey Pooling
32296183
Intra
PRKAG1 P54619 PAX6 Homo sapiens P26367
Y2H Array
32296183
Intra
PRKAG1 P54619 KEAP1 Homo sapiens Q14145
Y2H Prey Pooling
25416956
Intra
PRKAG1 P54619 KEAP1 Homo sapiens Q14145
Y2H Prey Pooling
32296183
Intra
PRKAG1 P54619 KEAP1 Homo sapiens Q14145
Validated Y2H
32296183
Intra
PRKAG1 P54619 KEAP1 Homo sapiens Q14145
Y2H Array
25416956
Intra
PRKAG1 P54619 KEAP1 Homo sapiens Q14145
Y2H Array
32296183
Intra
PRKAG1 P54619 C1orf94 Homo sapiens Q6P1W5
Validated Y2H
25416956
Intra
PRKAG1 P54619 C1orf94 Homo sapiens Q6P1W5
Y2H Array
25416956
Cross: Cross-species interaction Intra: Intraspecies interaction

Recombinant PRKAG1 Proteins

Cat. No. Nom du produit Accession Pureté
HY-P76141 AMPK gamma 1/beta 1/alpha 1 Heterotrimer Protein, Human (Active, sf9, His-GST) P54619-1 (M1-P331)&Q9Y478 (M1-I270)&Q13131-1 (M1-Q559) ≥ 90%, as determined by reducing SDS-PAGE.
HY-P76142 AMPK gamma 1/beta 1/alpha 2 Heterotrimer Protein, Human (Active, sf9, His-GST) P54619-1 (M1-P331)&Q9Y478 (M1-I270)&P54646 (M1-R552) ≥ 90%, as determined by reducing SDS-PAGE.
HY-P76143 AMPK gamma 1/beta 2/alpha 1 Heterotrimer Protein, Human (Active, sf9, His-GST) P54619-1 (M1-P331)&O43741 (M1-I272)&Q13131-1 (M1-Q559) ≥ 90%, as determined by reducing SDS-PAGE.
HY-P76144 AMPK gamma 1/beta 2/alpha 2 Heterotrimer Protein, Human (Active, sf9, His-GST) P54619-1 (M1-P331)&O43741 (M1-I272)&P54646 (M1-R552) ≥ 90%, as determined by reducing SDS-PAGE.

PRKAG1 Anticorps

Cat. No. Nom du produit Application Reactivity
HY-P80543 AMPK gamma 1 Antibody (YA621) WB, ICC/IF, IP Human
HY-P80543A AMPK gamma 1 Antibody (YA621)(PBS only) WB, ICC/IF, IP Human
HY-P84393 AMPK gamma 1 Antibody (YA4090) IHC-P, ICC/IF, FC, ELISA Human
HY-P84393A AMPK gamma 1 Antibody (YA4090)(PBS only) IHC-P, ICC/IF, FC, ELISA Human
HY-P85706 AMPK gamma 1 Antibody (YA5398) IHC-P, WB Human, Mouse, Rat

Related Diseases

Diseases Alias
Malignant Essential Hypertension

  • Accelerated Essential Hypertension
Trichuriasis

  • Whipworm Infection

  • Whipworm Disease

  • Trichocephaliasis

  • Infection By Trichuris Trichura

  • Trichuriasis Infection

  • Trichuris Trichiura Infection

  • Infection By Trichuris Trichiura

  • Trichocephalosis

  • Infection By Trichocephalus Trichiura

  • Infection By Trichuris

  • Infection By Trichuris Trichiuria

  • Trichuriosis

  • Whipworm

  • Infection Due To Trichuris Trichiura

  • Trichocephalus Infestation

  • Trichuris Infestation

  • Whipworm Infestation
Dysferlinopathy

  • Dystrophinopathy
Wolff-Parkinson-White Syndrome

  • Wolff-Parkinson-White Pattern

  • Wpw Syndrome

  • Anomalous Atrioventricular Excitation

  • Anomalous A-V Excitation

  • Ventricular Pre-Excitation With Arrhythmia

  • WPWS

  • Ventricular Familial Preexcitation Syndrome

  • Preexcitation Syndrome

  • Ventricular Preexcitation

  • Wpw - [Wolff-Parkinson- White] Syndrome

  • Pre-Excitation Syndrome
Diseases Alias
Waldenstroem'S Macroglobulinemia

Waldenstroem'S Macroglobulinemia

Waldenstroem'S Macroglobulinemia

Macroglobulinemia Of Waldenstrom

Lymphoplasmacytic Lymphoma With Igm Gammopathy

Lymphoplasmacytic Lymphoma

Waldenstroem'S Macroglobulinemia

Waldenstroem'S Macroglobulinemia

Macroglobulinemia Of Waldenstrom

Lymphoplasmacytic Lymphoma With Igm Gammopathy

Lymphoplasmacytic Lymphoma

Related Agents

  • Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (1)
Cat. No. Nom du produit
Effect
Pureté
Rare Diseases
HY-RS11109 PRKAG1 Human Pre-designed siRNA Set A Pre-designed Set / Unkown

Orthologs Information

Species Symbol Source ID
Mus musculus PRKAG1 MGD MGI:108411
Macaca mulatta PRKAG1 VGNC VGNC:76405
Rattus norvegicus PRKAG1 RGD RGD:3388
Felis catus PRKAG1 VGNC VGNC:97568
Canis familiaris PRKAG1 VGNC VGNC:44972
Bos taurus PRKAG1 VGNC VGNC:33322
Others PRKAG1 NCBI