HPPD-IN-10

HPPD-IN-10 (compound A3) potently inhibits Arabidopsis thaliana 4-hydroxyphenylpyruvate dioxygenase (HPPD), with an IC₅₀ value of 0.06 μM^[1].
HPPD-IN-10 (10-100 μg/mL; 5-day incubation) exhibits strong pre-emergence herbicidal activity against germinated Portulaca oleracea seeds and moderate activity against germinated Echinochloa crusgalli seeds; at the concentration of 100 μg/mL, its root inhibition rate on Portulaca oleracea reaches 83.4% and shoot inhibition rate reaches 92.2%^[1].
HPPD-IN-10 binds to AtHPPD through bidentate chelation with Co²⁺, π−π interactions with Phe381 and Phe424, hydrophobic interactions with Gln293 and Leu265, and hydrogen bonding with Asn282^[1].
HPPD-IN-10 binds tightly to wild-type Arabidopsis thaliana HPPD with a K_d value of 0.265 μM; however, its binding affinity to the AtHPPD^N282A mutant is significantly reduced, which confirms that Asn282 is a key binding site^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.