Click chemistry to construct fluorescent oligonucleotides for DNA sequencing

  • J Org Chem. 2003 Jan 24;68(2):609-12. doi: 10.1021/jo026615r.
Tae Seok Seo  1 ,  Zengmin Li ,  Hameer Ruparel ,  Jingyue Ju
Affiliations
  • 1. Columbia Genome Center, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA.
PMID: 12530893 DOI: 10.1021/jo026615r
Abstract

"Click chemistry" 1,3-dipolar cycloaddition between alkynyl 6-carboxyfluorescein (FAM) and azido-labeled single-stranded (ss) DNA was carried out under aqueous conditions to produce FAM-labeled ssDNA in quantitative yield. The FAM-labeled ssDNA was successfully used as a primer to produce DNA Sequencing products with single-base resolution in a capillary electrophoresis DNA sequencer with laser-induced fluorescence detection.

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