CS790AM  (Synonyms: Coppersensor 790 acetoxymethyl ester)

CS790AM (Coppersensor 790 acetoxymethyl ester) is a cell-permeable, Cu⁺-targeted near-infrared fluorescent probe (λ_abs=760 nm, λ_em=790 nm) applicable to live cells. CS790AM can cross lipophilic cell membranes, and is converted into negatively charged CS790 under the action of intracellular esterases to be retained, thus enabling highly sensitive, reversible "turn-on" detection of labile Cu⁺ pools in live cells and mice. CS790AM possesses excellent biocompatibility and selectivity, avoids interference from other metal ions, shows no obvious toxicity, and can be rapidly cleared. CS790AM allows long-term longitudinal monitoring of individual mice, visualizes copper levels in internal organs and isolated livers, and effectively evaluates abnormal copper accumulation in Wilson's disease models (Atp7b^-/-) as well as dynamic changes after chelator treatment. CS790AM can be used for research on Wilson's disease and related copper metabolic disorders^[1][2][3].

CS790AM, a Cy7-based fluorescent probe, produces a 17-fold fluorescence turn-on response upon binding to Cu⁺ in cell-free conditions^[1].
CS790AM (2 μM; 15 min at 37 °C) reversibly detects fluctuations in labile copper levels in HEK 293T cells, with a significant increase in mean fluorescence intensity in copper-overloaded cells that is reversed by copper chelation^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CS790AM (0.1 mM; i.p.; single dose) detects dynamic fluctuations in labile Cu⁺ levels in healthy SKH-1 mice, producing a ~60% fluorescence increase in copper-overloaded mice and reduced signal in copper-depleted mice, with reversible responses to chelation^[2].
CS790AM (0.1 mM; i.p.; single dose) detects aberrantly elevated labile Cu⁺ levels in Atp7b^-/^- Wilson disease model mice, producing significantly higher fluorescence signal than in wild-type mice at 30 minutes post-injection, with signal reducible by copper chelation^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1105.27

C₅₆H₇₀BrN₃O₉S₃

1416808-87-1

O=C(CC[N+]1=C(C(C)(C2=CC=CC=C21)C)/C=C/C(CCC/C3=C\C=C4N(C5=CC=CC=C5C\4(C)C)CCC(OCOC(C)=O)=O)=C3OC6=CC(SCCCSC)=C(C=C6)NCCCSC)OCOC(C)=O.[Br-]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Ackerman C M, et al. Analytical methods for imaging metals in biology: from transition metal metabolism to transition metal signaling[J]. Analytical chemistry, 2017, 89(1): 22-41.

  [2]. Hirayama T, et al. Near-infrared fluorescent sensor for in vivo copper imaging in a murine Wilson disease model. Proc Natl Acad Sci U S A. 2012;109(7):2228-2233.

  [3]. Cotruvo JA Jr, et al. Synthetic fluorescent probes for studying copper in biological systems. Chem Soc Rev. 2015;44(13):4400-4414.