Dynamin 1 Antibody (YA779)
(Synonyms: DNM, DNM1, Dynamin-1, Dynamin, Dynamin I)
Based on 1 Customer Validation
Dynamin 1 Antibody (YA779) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Dynamin 1.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
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| Dilution Ratio | 1:500-1:2000 | 1:50-1:200 | 1:50 |
Product Details
Dynamin 1 Antibody (YA779) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Dynamin 1.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 100 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 97 kDa
Entrez Gene: 1759 Human ; 13429 Mouse ; 140694 Rat
SwissProt: Q05193 Human ; P39053 Mouse ; P21575 Rat
OMIM: 616346 Human
Synthetic peptide corresponding to Human Dynamin 1.AA range:555-770.
Endogenous
Protein A affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from Hela (lane 2(20μg), SH-SY5Y (lane 3(20μg), NIH/3T3 (lane 4(20μg), using Dynamin 1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Dynamin 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Dynamin 1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of HeLa cells labeling Dynamin 1 with Dynamin 1 Antibody (HY-P80390) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Dynamin 1 Antibody (HY-P80390) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). -
Immunocytochemistry analysis of Siha cells labeling Dynamin 1 with Dynamin 1 Antibody (HY-P80390) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Dynamin 1 Antibody (HY-P80390) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Background
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Function
Dynamin 1 catalyzes the hydrolysis of GTP and utilizes this energy to mediate vesicle scission and participates in many forms of endocytosis, such as clathrin-mediated endocytosis or synaptic vesicle endocytosis as well as rapid endocytosis (RE). Associates to the membrane, through lipid binding, and self-assembles into rings and stacks of interconnected rings through oligomerization to form a helical polymer around the vesicle membrane leading to constriction of invaginated coated pits around their necks. Self-assembly of the helical polymer induces membrane tubules narrowing until the polymer reaches a length sufficient to trigger GTP hydrolysis. Depending on the curvature imposed on the tubules, membrane detachment from the helical polymer upon GTP hydrolysis can cause spontaneous hemifission followed by complete fission. May play a role in regulating early stages of clathrin-mediated endocytosis in non-neuronal cells through its activation by dephosphorylation via the signaling downstream of EGFR. Controls vesicle size at a step before fission, during formation of membrane pits, at hippocampal synapses. Controls plastic adaptation of the synaptic vesicle recycling machinery to high levels of activity. Mediates rapid endocytosis (RE), a Ca(2+)-dependent and clathrin- and K(+)-independent process in chromaffin cells. Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Through its interaction with DNAJC6, acts during the early steps of clathrin-coated vesicle (CCV) formation[1][2][3][4][5][6][7][8][9][10][11].
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Subcellular Localization
Cell membrane; Membrane, clathrin-coated pit; Cytoplasmic vesicle; Presynapse; Cytoplasmic vesicle, secretory vesicle, chromaffin granule
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Subunit
Homodimer; homodimerization is mediated by the dynamin-type G domain which promotes assembly-stimulated GTPase activity (PubMed:20428113, PubMed:26612256).
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SwissProt ID
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Synonyms
DNM, DNM1, Dynamin-1, Dynamin, Dynamin I
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Research Field
Signal Transduction
Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
[1]. Soulet F, et al. SNX9 regulates dynamin assembly and is required for efficient clathrin-mediated endocytosis. Mol Biol Cell. 2005 Apr;16(4):2058-67. [Content Brief]
[2]. Chappie JS, et al. G domain dimerization controls dynamin's assembly-stimulated GTPase activity. Nature. 2010 May 27;465(7297):435-40. [Content Brief]
[3]. Srinivasan S, et al. A noncanonical role for dynamin-1 in regulating early stages of clathrin-mediated endocytosis in non-neuronal cells. PLoS Biol. 2018 Apr;16(4):e2005377. [Content Brief]
[4]. van der Bliek AM, et al. Mutations in human dynamin block an intermediate stage in coated vesicle formation. J Cell Biol. 1993 Aug;122(3):553-63. [Content Brief]
[5]. Lin HC, et al. Regulation of dynamin I GTPase activity by G protein betagamma subunits and phosphatidylinositol 4,5-bisphosphate. J Biol Chem. 1996 Nov 8;271(45):27979-82. [Content Brief]
[6]. Muhlberg AB, et al. Domain structure and intramolecular regulation of dynamin GTPase. EMBO J. 1997 Nov 17;16(22):6676-83. [Content Brief]
[7]. Kong L, et al. Cryo-EM of the dynamin polymer assembled on lipid membrane. Nature. 2018 Aug;560(7717):258-262. [Content Brief]
[8]. Hinshaw JE, et al. Dynamin self-assembles into rings suggesting a mechanism for coated vesicle budding. Nature. 1995 Mar 9;374(6518):190-2. [Content Brief]
[9]. Scaife R, et al. Dual function C-terminal domain of dynamin-1: modulation of self-assembly by interaction of the assembly site with SH3 domains. Biochemistry. 1998 Dec 22;37(51):17673-9. [Content Brief]
[10]. Bashkirov PV, et al. GTPase cycle of dynamin is coupled to membrane squeeze and release, leading to spontaneous fission. Cell. 2008 Dec 26;135(7):1276-86. [Content Brief]
[11]. Newmyer SL, et al. Auxilin-dynamin interactions link the uncoating ATPase chaperone machinery with vesicle formation. Dev Cell. 2003 Jun;4(6):929-40. [Content Brief]
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