2,3-Diaminophenazine
Based on 1 Customer Validation
2,3-Diaminophenazine (2,3-Phenazinediamine) is an orally active amino derivative of phenazine. 2,3-Diaminophenazine can intercalate into DNA. 2,3-Diaminophenazine triggers photochemical reactions. 2,3-Diaminophenazine inhibits vascular hypertrophy and tissue AGE deposition in diabetic rats. 2,3-Diaminophenazine can be used for luminescence and diabetes research.
For research use only. We do not sell to patients.
- Purity: 99.84%
- CAS No.: 655-86-7
- Formula: C12H10N4
- Molecular Weight:210.24
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
8.8 μg/mL
Compound: 1
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Antiproliferative activity against human A549 cells
Antiproliferative activity against human A549 cells
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[PMID: 25497130] |
| HCT-116 | IC50 |
6 μg/mL
Compound: 1
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Antiproliferative activity against human HCT116 cells
Antiproliferative activity against human HCT116 cells
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[PMID: 25497130] |
| Huh-7 | CC50 |
>10 μg/mL
Compound: 1
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Cytotoxic concentration of compound HC40 cells determined by using MTT assay
Cytotoxic concentration of compound HC40 cells determined by using MTT assay
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[PMID: 10866369] |
| Huh-7 | IC50 |
4.03 μg/mL
Compound: 1
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Anti-Hepatitis C Viral IRES activity of compound by measured as Fluci signal in HC40 cells
Anti-Hepatitis C Viral IRES activity of compound by measured as Fluci signal in HC40 cells
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[PMID: 10866369] |
2,3-Diaminophenazine shows weak intercalative binding to double-stranded DNA with a binding constant Kb of 3.5 mM[3].
2,3-Diaminophenazine (13 μM) combined with visible light irradiation resulted in a 13-fold decrease in the lethal concentration 50 (LC50) in human skin fibroblasts (Hs-27) compared with dark conditions, indicating significant phototoxicity[3].
2,3-Diaminophenazine effectively inhibits the formation of AGEs with an IC50 range of 30-40 µM (HPLC and 14C-lysine method) or < 10 µM (ELISA method)[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 655-86-7
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Appearance Solid
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Molecular Weight 210.24
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Formula C12H10N4
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Color Yellow to orange
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SMILES
NC1=CC2=NC3=CC=CC=C3N=C2C=C1N
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Synonyms
2,3-Phenazinediamine; NNC39-0028
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 5.83 mg/mL (27.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Xiaoyue He, et al. Selective self-assembly of 2,3-diaminophenazine molecules on MoSe 2 mirror twin boundaries. Nat Commun. 2019 Jun 28;10(1):2847. [Content Brief]
[2]. Feng Zhang, et al. An enzymatic ratiometric fluorescence assay for 6-mercaptopurine by using MoS 2 quantum dots. Mikrochim Acta. 2018 Nov 10;185(12):540. [Content Brief]
[3]. Fu PK, et al. Photoinduced DNA cleavage and cellular damage in human dermal fibroblasts by 2,3-diaminophenazine. Photochem Photobiol. 2005 Jan-Feb;81(1):89-95. [Content Brief]
[4]. Soulis T, et al. A novel inhibitor of advanced glycation end-product formation inhibits mesenteric vascular hypertrophy in experimental diabetes. Diabetologia. 1999 Apr;42(4):472-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.7565 mL | 23.7823 mL | 47.5647 mL | 118.9117 mL |
| 5 mM | 0.9513 mL | 4.7565 mL | 9.5129 mL | 23.7823 mL | |
| 10 mM | 0.4756 mL | 2.3782 mL | 4.7565 mL | 11.8912 mL | |
| 15 mM | 0.3171 mL | 1.5855 mL | 3.1710 mL | 7.9274 mL | |
| 20 mM | 0.2378 mL | 1.1891 mL | 2.3782 mL | 5.9456 mL | |
| 25 mM | 0.1903 mL | 0.9513 mL | 1.9026 mL | 4.7565 mL |
2,3-Diaminophenazine Related Classifications
HY-W020086 Related Classifications
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