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Cat. No.: HY-151914

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Antitumor agent-82

Antitumor agent-82 (compound 6g) is a potent anti-tumor agent. Antitumor agent-82 shows anti-proliferative activity. Antitumor agent-82 induces cell Autophagy by the ATG5/ATG7 signaling pathway.

For research use only. We do not sell to patients.

CAS No.: 3037605-45-8
Formula: C₃₂H₄₂N₆
Molecular Weight:510.72

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (275 KB)

Antitumor agent-82 Chemical Structure

CAS No.: 3037605-45-8

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
786-0	IC₅₀	2.71 μM Compound: 6g	Antiproliferative activity against human 786-0 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human 786-0 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 36459757]
A-375	IC₅₀	11.5 μM Compound: 6g	Antiproliferative activity against human A-375 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human A-375 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 36459757]
BGC-823	IC₅₀	24.8 μM Compound: 6g	Antiproliferative activity against human BGC-823 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human BGC-823 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 36459757]
CWR22R	IC₅₀	3.86 μM Compound: 6g	Antiproliferative activity against human 22Rv1 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human 22Rv1 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 36459757]
ECa-109 cell line	IC₅₀	8.62 μM Compound: 6g	Antiproliferative activity against human Eca-109 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human Eca-109 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 36459757]
HCT-116	IC₅₀	4.57 μM Compound: 6g	Antiproliferative activity against human HCT-116 cells expressing ATG5-FLAG assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay Antiproliferative activity against human HCT-116 cells expressing ATG5-FLAG assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay	[PMID: 36459757]
HCT-116	IC₅₀	4.71 μM Compound: 6g	Antiproliferative activity against p53 knockout human HCT-116 cells assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay Antiproliferative activity against p53 knockout human HCT-116 cells assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay	[PMID: 36459757]
HCT-116	IC₅₀	4.97 μM Compound: 6g	Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay	[PMID: 36459757]
HCT-116	IC₅₀	5.09 μM Compound: 6g	Antiproliferative activity against human HCT-116 cells expressing ATG7-FLAG assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay Antiproliferative activity against human HCT-116 cells expressing ATG7-FLAG assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay	[PMID: 36459757]
HCT-116	IC₅₀	7.36 μM Compound: 6g	Antiproliferative activity against ATG5 knockout human HCT-116 cells assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay Antiproliferative activity against ATG5 knockout human HCT-116 cells assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay	[PMID: 36459757]
HCT-116	IC₅₀	7.89 μM Compound: 6g	Antiproliferative activity against ATG7 knockout human HCT-116 cells assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay Antiproliferative activity against ATG7 knockout human HCT-116 cells assessed as inhibition of cell growth measured after 24 hrs by CCK-8 assay	[PMID: 36459757]
HT-29	IC₅₀	2.02 μM Compound: 6g	Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 36459757]
KB	IC₅₀	8.12 μM Compound: 6g	Antiproliferative activity against human KB cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human KB cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 36459757]
MCF7	IC₅₀	13.5 μM Compound: 6g	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 36459757]
SK-OV-3	IC₅₀	10.6 μM Compound: 6g	Antiproliferative activity against human SK-OV-3 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human SK-OV-3 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 36459757]

In Vitro

Antitumor agent-82 (compound 6g) (0-100 μM; 48 h) shows anti-proliferative activity with IC₅₀s of 24.8, 13.5, 11.5, 2.71, 2.02, 4.53 μM for BGC-823, MCF7, A375, 786-O, HT-29, Blu-87 cells, respectively^[1].
Antitumor agent-82 (0-4 μM; 0-7 days) inhibits cell growth in a dose and time-dependent manner against HCT116 cells^[1].
Antitumor agent-82 (0-5 μM; 0-60 h) activates autophagy by the ATG5/ATG7 signaling pathway in HCT116 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

BGC-823, MCF7, A375, 786-O, HT-29, Blu-87 cells
Concentration:

0-100 µM
Incubation Time:

48 h
Result:

Showed antiproliferative activity with IC₅₀s of 24.8, 13.5, 11.5, 2.71, 2.02, 4.53 µM for BGC-823, MCF7, A375, 786-O, HT-29, Blu-87 cells, respectively.

Western Blot Analysis^[1]

Cell Line:

HCT116 cells
Concentration:

0-5 µM
Incubation Time:

0-60 h
Result:

Induced autophagy with no influences on the expression of caspase-3, cleaved caspase-3 and p53 protein, significantly increased the expression of LC3-II and p62.

In Vivo

Antitumor agent-82 (45 mg/kg; i.p.; every two days for 16 days) shows anti-cancer activity for mouse^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

6-week-old male BALB/c mice^[1]
Dosage:

45 mg/kg
Administration:

I.p.; every two days for 16 days
Result:

Reduced tumor volume and resulted in a considerable reduction of the tumor weight of 69.69%.

Chemical Information

CAS No. 3037605-45-8
Molecular Weight 510.72
Formula C₃₂H₄₂N₆
SMILES

CN1CCN(CC1)CC2=NC(CN3CCN(CC3)C)=C4C(C5=C(N4CCCC6=CC=CC=C6)C=CC=C5)=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Ao J,et al. Design, synthesis and pharmacological evaluation of β-carboline derivatives as potential antitumor agent via targeting autophagy. Eur J Med Chem. 2022 Nov 26;246:114955. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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