Biotin-(Oaa)3-epoxomicin
Based on 1 Customer Validation
Biotin-(Oaa)3-epoxomicin is a biotin-labeled form of Epoxomicin (HY-13821), prepared by conjugating Epoxomicin with biotin via three hydrophilic oxaacetyl amino acid (Oaa) linkers. Biotin-(Oaa)3-epoxomicin is primarily used in proteomic studies for the capture, identification and target validation of proteasome complexes, to determine the intracellular targets of epoxomicin. Epoxomicin acts as a proteasome inhibitor and NF-κB inhibitor, which effectively blocks inflammatory responses in mouse ear edema assays. It inhibits proteasome activity via covalent binding to catalytic subunits including LMP7, X, MECL1 and Z, with the strongest inhibitory effect on chymotrypsin-like activity, and does not interfere with non-proteasomal proteases such as trypsin and papain.
For research use only. We do not sell to patients.
- Purity: 96.46%
- CAS No.: 247068-92-4
- Formula: C54H95N9O11S
- Molecular Weight:1078.45
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Epoxomicin (5-8 μM; 4 hr) covalently binds to the LMP7, MECL1, Z, and X catalytic β subunits of the proteasome in EL4 murine thymoma cells and LCL 721.45/LCL 721.174 human B cell lymphoma cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Biotin-{Oaa}-{Oaa}-{Oaa}-Ile-Ile-Thr-{Leu((2R)-2-methyloxiranyl)}
Biotin-{Oaa}-{Oaa}-{Oaa}-IIT-{Leu((2R)-2-methyloxiranyl)}
Chemical Information
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CAS No. 247068-92-4
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Appearance Solid
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Molecular Weight 1078.45
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Formula C54H95N9O11S
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Color White to off-white
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : ≥ 100 mg/mL (92.73 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Purity & Documentation
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Data Sheet (270 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Meng L, et al. Epoxomicin, a potent and selective proteasome inhibitor, exhibits in vivo antiinflammatory activity. Proc Natl Acad Sci U S A. 1999;96(18):10403-10408. [Content Brief]
[2]. Hanada M, et al. Epoxomicin, a new antitumor agent of microbial origin. J Antibiot (Tokyo). 1992;45(11):1746-1752. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.9273 mL | 4.6363 mL | 9.2726 mL | 23.1814 mL |
| 5 mM | 0.1855 mL | 0.9273 mL | 1.8545 mL | 4.6363 mL | |
| 10 mM | 0.0927 mL | 0.4636 mL | 0.9273 mL | 2.3181 mL | |
| 15 mM | 0.0618 mL | 0.3091 mL | 0.6182 mL | 1.5454 mL | |
| 20 mM | 0.0464 mL | 0.2318 mL | 0.4636 mL | 1.1591 mL | |
| 25 mM | 0.0371 mL | 0.1855 mL | 0.3709 mL | 0.9273 mL | |
| 30 mM | 0.0309 mL | 0.1545 mL | 0.3091 mL | 0.7727 mL | |
| 40 mM | 0.0232 mL | 0.1159 mL | 0.2318 mL | 0.5795 mL | |
| 50 mM | 0.0185 mL | 0.0927 mL | 0.1855 mL | 0.4636 mL | |
| 60 mM | 0.0155 mL | 0.0773 mL | 0.1545 mL | 0.3864 mL | |
| 80 mM | 0.0116 mL | 0.0580 mL | 0.1159 mL | 0.2898 mL |
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