2. Immunology/Inflammation
  3. Transmembrane Glycoprotein
  4. EP9

EP9 is a peptide targeting CD63. EP9 binds specifically to the extracellular region of CD63, including a groove in the large extracellular loop (EC2) or the extracellular end of CD63’s central cavity, triggering endocytosis of decorated nanoemulsions/liposomes into cells. EP9 promotes cellular uptake of decorated nanoemulsions/liposomes into activated cardiac fibroblasts and epicardial stromal cells via caveolae and/or clathrin-coated pits. EP9 can be used for the research of myocardial infarction, cardiac fibrosis.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

EP9

EP9 Chemical Structure

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EP9 Related Antibodies

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  • Purity & Documentation

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Description

EP9 is a peptide targeting CD63. EP9 binds specifically to the extracellular region of CD63, including a groove in the large extracellular loop (EC2) or the extracellular end of CD63’s central cavity, triggering endocytosis of decorated nanoemulsions/liposomes into cells. EP9 promotes cellular uptake of decorated nanoemulsions/liposomes into activated cardiac fibroblasts and epicardial stromal cells via caveolae and/or clathrin-coated pits. EP9 can be used for the research of myocardial infarction, cardiac fibrosis[1].

In Vitro

EP9 (5 µg; 30 min) binds specifically to mouse cardiac fibroblasts and EpiSC, human primary cardiac fibroblasts, and normal human dermal fibroblasts, with the highest binding affinity for mouse EpiSC and human primary cardiac fibroblasts[1].
EP9 (1 mM; 1 h) specifically binds to the tetraspanin CD63, a transmembrane protein on the surface of normal human dermal fibroblasts, as confirmed by significant enrichment in EP9 probe samples relative to control MUT probe samples[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EP9 Related Antibodies
In Vivo

EP9 (25 µg per 100 µL EP9-PFC-NE; 150 µL total; i.v.; single dose) mediates selective accumulation of conjugated PFC-NE in infarcted myocardial tissue, with preferential uptake by cardiac fibroblasts and epicardial stromal cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6J (male, 8-12 weeks old, 20-25 g, myocardial infarction induced by 50 min left anterior descending coronary artery ligation followed by reperfusion)[1]
Dosage: 25 µg EP9 per 100 µL PFC-NE; 150 µL EP9-PFC-NE
Administration: i.v.; single dose
Result: Localized a strong 19F signal specifically to the infarcted anterior wall of the left ventricle on in vivo MRI, with a signal-to-noise ratio significantly higher than that of the mutated peptide control.
Declined plasma 19F signal with a half-life of ~20 hours.
Showed a transmural 19F signal in the infarcted area that increased between 12 hours and 24 hours post-injection via ex vivo MRI.
Revealed EP9-PFC-NE uptake primarily by cardiac fibroblasts and epicardial stromal cells, with no detectable uptake in macrophages, cardiomyocytes, or endothelial cells via transmission electron microscopy.
Demonstrated intracellular particles had an average size of 118 nm, matching the pre-injection particle size of 124 nm.
Molecular Weight

1297.64

Formula

C56H100N18O13S2
Appearance

Solid

Color

White to off-white

Sequence

Ac-Lys-Leu-Met-Leu-Pro-Arg-Pro-Gly-Gly-Gly-Lys-Cys-NH2
Sequence Shortening

Ac-KLMLPRPGGGKC-NH2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (77.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.7706 mL 3.8531 mL 7.7063 mL
5 mM 0.1541 mL 0.7706 mL 1.5413 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.7706 mL 3.8531 mL 7.7063 mL 19.2657 mL
5 mM 0.1541 mL 0.7706 mL 1.5413 mL 3.8531 mL
10 mM 0.0771 mL 0.3853 mL 0.7706 mL 1.9266 mL
15 mM 0.0514 mL 0.2569 mL 0.5138 mL 1.2844 mL
20 mM 0.0385 mL 0.1927 mL 0.3853 mL 0.9633 mL
25 mM 0.0308 mL 0.1541 mL 0.3083 mL 0.7706 mL
30 mM 0.0257 mL 0.1284 mL 0.2569 mL 0.6422 mL
40 mM 0.0193 mL 0.0963 mL 0.1927 mL 0.4816 mL
50 mM 0.0154 mL 0.0771 mL 0.1541 mL 0.3853 mL
60 mM 0.0128 mL 0.0642 mL 0.1284 mL 0.3211 mL
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EP9 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

EP9EP 9EP-9Transmembrane Glycoproteinmyocardial infarctionnormal human dermal fibroblastsmouse cardiac fibroblastshuman primary cardiac fibroblastscaveolaeepicardial stromal cellsactivated cardiac fibroblastsCD63cardiac fibrosisclathrin-coated pitsInhibitorinhibitorinhibit

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