2. Immunology/Inflammation
  3. Transmembrane Glycoprotein
  4. EP9

EP9 is a 7-amino acid peptide targeting CD63. EP9 binds specifically to the extracellular region of CD63, including a groove in the large extracellular loop (EC2) or the extracellular end of CD63’s central cavity, triggering endocytosis of decorated nanoemulsions/liposomes into cells. EP9 promotes cellular uptake of decorated nanoemulsions/liposomes into activated cardiac fibroblasts and epicardial stromal cells via caveolae and/or clathrin-coated pits. EP9 can be used for the research of myocardial infarction, cardiac fibrosis.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

EP9

EP9 Chemical Structure

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EP9 Related Antibodies

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Description

EP9 is a 7-amino acid peptide targeting CD63. EP9 binds specifically to the extracellular region of CD63, including a groove in the large extracellular loop (EC2) or the extracellular end of CD63’s central cavity, triggering endocytosis of decorated nanoemulsions/liposomes into cells. EP9 promotes cellular uptake of decorated nanoemulsions/liposomes into activated cardiac fibroblasts and epicardial stromal cells via caveolae and/or clathrin-coated pits. EP9 can be used for the research of myocardial infarction, cardiac fibrosis[1].

In Vitro

EP9 (5 µg; 30 min) binds specifically to mouse cardiac fibroblasts and EpiSC, human primary cardiac fibroblasts, and normal human dermal fibroblasts, with the highest binding affinity for mouse EpiSC and human primary cardiac fibroblasts[1].
EP9 (1 mM; 1 h) specifically binds to the tetraspanin CD63, a transmembrane protein on the surface of normal human dermal fibroblasts, as confirmed by significant enrichment in EP9 probe samples relative to control MUT probe samples[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EP9 Related Antibodies
In Vivo

EP9 (25 µg per 100 µL EP9-PFC-NE; 150 µL total; i.v.; single dose) mediates selective accumulation of conjugated PFC-NE in infarcted myocardial tissue, with preferential uptake by cardiac fibroblasts and epicardial stromal cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6J (male, 8-12 weeks old, 20-25 g, myocardial infarction induced by 50 min left anterior descending coronary artery ligation followed by reperfusion)[1]
Dosage: 25 µg EP9 per 100 µL PFC-NE; 150 µL EP9-PFC-NE
Administration: i.v.; single dose
Result: Localized a strong 19F signal specifically to the infarcted anterior wall of the left ventricle on in vivo MRI, with a signal-to-noise ratio significantly higher than that of the mutated peptide control.
Declined plasma 19F signal with a half-life of ~20 hours.
Showed a transmural 19F signal in the infarcted area that increased between 12 hours and 24 hours post-injection via ex vivo MRI.
Revealed EP9-PFC-NE uptake primarily by cardiac fibroblasts and epicardial stromal cells, with no detectable uptake in macrophages, cardiomyocytes, or endothelial cells via transmission electron microscopy.
Demonstrated intracellular particles had an average size of 118 nm, matching the pre-injection particle size of 124 nm.
Molecular Weight

1297.64

Formula

C56H100N18O13S2
Sequence

Ac-Lys-Leu-Met-Leu-Pro-Arg-Pro-Gly-Gly-Gly-Lys-Cys-NH2
Sequence Shortening

Ac-KLMLPRPGGGKC-NH2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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EP9 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

EP9EP 9EP-9Transmembrane Glycoproteinmyocardial infarctionnormal human dermal fibroblastsmouse cardiac fibroblastshuman primary cardiac fibroblastscaveolaeepicardial stromal cellsactivated cardiac fibroblastsCD63cardiac fibrosisclathrin-coated pitsInhibitorinhibitorinhibit

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