6-AF488 tyramide
Based on 1 Customer Validation
6-AF488 tyramide is a bright, green fluorescent dye (Ex=496 nm, Em=524 nm). 6-AF488 tyramide is utilized as reporter fluorescent substrate of horseradish peroxidase (HRP)-catalyzed deposition for tyramide signal amplification (TSA). 5-FITC tyramide can be used for multiplex immunohistochemistry (mIHC).
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- Pureza: 99.68%
- No. CAS: 2435665-39-5
- Fòrmula: C29H23N3O11S2
- Peso molecular:653.64
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
6-AF488 tyramide assay[1].
a. Place tissues in per well in a 24-well plate, and it’s important to not let tissues sections dry.
b. Permeabilize tissues with 0.2 % Triton X-100 in PBS for 5-10 min.
c. Rinse with PBS-T for 5-10 min, and then incubate tissues in 1 % H2O2 for 20 min to block endogenous peroxidase activity.
d. Rinse with PBS three times, 5-10 min each, and then incubate in 1 % blocking solution (4 % normal donkey serum + PBS-T) in PBS for 1 h for to block nonspecific binding sites.
e. Apply the primary antibody working solution for 2 h at RT or overnight at 4 °C, and then rinse with PBS three times, 5-10 min each.
f. Apply the HRP-conjugated secondary goat anti rabbit antibody (1:100 in 1 % blocking solution) for 2 h at RT , and then rinse with PBS three times, 5-10 min each.
g. Activate TSA buffer with 0.0015 % H2O2, and then incubate tissues in 6-AF488 tyramide working solution (1: 100 in the activated TSA buffer) for 5-10 min at RT.
h. Rinse with PBS three times, 5-10 min each, and then mount sections on slides.
i. Place in slide holder in a nearly vertical position for 10 min to dry slides, and then briefly rinse slides with ddH2O following drying for 10 min.
j. Mount slides with mounting medium, we recommend using AntiFade Mounting Medium (with DAPI) (HY-K1047).
k. Fluorescence microscopy detection.
Note: it is suggested that the optimal concentration of 6-AF488 tyramide working solution according to actual conditions.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 2435665-39-5
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Appearance Solid
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Peso molecular 653.64
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Fòrmula C29H23N3O11S2
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Color Orange to red
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SMILES
O=C(O)C(C=C(C=C1)C(NCCC2=CC=C(C=C2)O)=O)=C1C(C3=C4C(S(=O)(O)=O)=C(N)C=C3)=C5C=CC(C(S(=O)([O-])=O)=C5O4)=[NH2+]
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
DMSO : 100 mg/mL (152.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5299 mL | 7.6495 mL | 15.2989 mL | 38.2474 mL |
| 5 mM | 0.3060 mL | 1.5299 mL | 3.0598 mL | 7.6495 mL | |
| 10 mM | 0.1530 mL | 0.7649 mL | 1.5299 mL | 3.8247 mL | |
| 15 mM | 0.1020 mL | 0.5100 mL | 1.0199 mL | 2.5498 mL | |
| 20 mM | 0.0765 mL | 0.3825 mL | 0.7649 mL | 1.9124 mL | |
| 25 mM | 0.0612 mL | 0.3060 mL | 0.6120 mL | 1.5299 mL | |
| 30 mM | 0.0510 mL | 0.2550 mL | 0.5100 mL | 1.2749 mL | |
| 40 mM | 0.0382 mL | 0.1912 mL | 0.3825 mL | 0.9562 mL | |
| 50 mM | 0.0306 mL | 0.1530 mL | 0.3060 mL | 0.7649 mL | |
| 60 mM | 0.0255 mL | 0.1275 mL | 0.2550 mL | 0.6375 mL | |
| 80 mM | 0.0191 mL | 0.0956 mL | 0.1912 mL | 0.4781 mL | |
| 100 mM | 0.0153 mL | 0.0765 mL | 0.1530 mL | 0.3825 mL |
6-AF488 tyramide Related Classifications
HY-D2874 Related Classifications
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