Direct Black 38
Based on 2 publication(s) in Google Scholar
Direct Black 38 (Chlorazol Black E) is a benzidine-based azo dye, a substrate of Enterococcus gallinarum, and is mainly used for textile dyeing. Direct Black 38 is biodegradable after being reduced by microbial enzymes (such as Enterococcus gallinarum azoreductase), breaking the azo bonds and releasing benzidine and 4-aminobiphenyl. Direct Black 38 can be used in environmental toxicology, wastewater treatment and carcinogenicity studies, but its metabolite benzidine is listed as a human carcinogen.
For research use only. We do not sell to patients.
- CAS No.: 1937-37-7
- Formula: C34H25N9Na2O7S2
- Molecular Weight:781.73
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Direct Black 38
More
Biological Activity
In the basal medium experiment, Direct Black 38 (25-100 mg/L; 24 h) is decolorized by 53-63% by Enterococcus gallinarum, releasing benzidine and 4-aminobiphenyl[1].
In the Luria broth experiment, Direct Black 38 (20-250 mg/L; 48 h) achieves a decolorization rate of 71-85%, with higher biomass efficiency, in line with Monod kinetics (Vmax=12.8 mg·L-1·h-1), Ks=490.6 mg·L-1)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1937-37-7
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Appearance Solid
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Molecular Weight 781.73
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Formula C34H25N9Na2O7S2
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Color Light brown to black
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SMILES
O=S(C1=C(/N=N/C2=CC=C(C3=CC=C(/N=N/C4=CC=C(N)C=C4N)C=C3)C=C2)C(N)=C5C(O)=C(/N=N/C6=CC=CC=C6)C(S(=O)(O[Na])=O)=CC5=C1)(O[Na])=O
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Synonyms
Chlorazol Black E; Ferristatin II disodium; C.I. 30235
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (2)
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Journal Impact Factor
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Most Recent
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Cell Metab
Ferroplasticity drives social isolation-induced anxiety via a ventral hippocampal iron-α-synuclein axis. [Abstract]2026 Jan 27:S1550-4131(25)00586-8. PMID: 41605214 -
Int J Mol Sci
CD71-Mediated Effects of Soluble Vasorin on Tumor Progression, Angiogenesis and Immunosuppression. [Abstract]2025 May 20;26(10):4913. PMID: 40430053
Solvent & Solubility
DMSO : 25 mg/mL (31.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 5 mg/mL (6.40 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Purity & Documentation
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Data Sheet (272 KB)
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SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Portuguese - PT (419 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.2792 mL | 6.3961 mL | 12.7921 mL | 31.9804 mL |
| 5 mM | 0.2558 mL | 1.2792 mL | 2.5584 mL | 6.3961 mL | |
| DMSO | 10 mM | 0.1279 mL | 0.6396 mL | 1.2792 mL | 3.1980 mL |
| 15 mM | 0.0853 mL | 0.4264 mL | 0.8528 mL | 2.1320 mL | |
| 20 mM | 0.0640 mL | 0.3198 mL | 0.6396 mL | 1.5990 mL | |
| 25 mM | 0.0512 mL | 0.2558 mL | 0.5117 mL | 1.2792 mL | |
| 30 mM | 0.0426 mL | 0.2132 mL | 0.4264 mL | 1.0660 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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