L57 acetate
Based on 1 Customer Validation
L57 acetate is a Low-density lipoprotein receptor-related protein 1 (LRP1)-binding peptide. L57 acetate exhibits high affinity to LRP1 with Ki of 45 nM. L57 acetate exhibits blood-brain barrier (BBB) permeability and plasma stability. L57 acetate can be utilized as the carrier for CNS drug delivery.
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- Pureza: 99.01%
- Fòrmula: C120H175N31O27.xC2H4O2
- Peso molecular:2483.87 (free base)
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Almacenamiento:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
L57 (EC50-determining concentrations; 1 h) acetate selectively binds to recombinant human LRP1(CL4)-Fc with an EC50 of 45 nM in a plate ELISA assay[1].
Fluorescein-labeled L57 (3-30 μM; 4 h, 1 min) exhibits rapid, concentration-dependent, LRP1-mediated uptake in primary rat BMVECs and astrocytes, with significantly higher uptake efficiency than the reference peptide at matching concentrations, and shows limited uptake in LRP1-deficient PEA 10 cells[2].
Fluorescein-labeled L57 (3-30 μM; 4 h) shows excellent biocompatibility with no cytotoxicity observed in primary rat BMVECs or LRP1-deficient PEA 10 cells at concentrations up to 30 μM after 4 h of incubation[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Primary rat brain microvascular endothelial cells (BMVECs), LRP1-deficient PEA 10 mouse embryonic fibroblasts
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Concentration:3 μM, 10 μM, 30 μM
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Incubation Time:4 h
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Result:Resulted in high cell viability in both BMVECs and PEA 10 cells, with metabolic activity comparable to vehicle control conditions at all tested concentrations.
L57 (i.v.; single dose) acetate results in modest brain delivery in male C57BL/6J mice, with a % input value of 0.042[1].
L57 acetate demonstrates significant blood-brain barrier permeability and high LRP1 binding affinity in healthy mice, supporting its potential as a CNS drug delivery vector[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Peso molecular 2483.87 (free base)
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Fòrmula C120H175N31O27.xC2H4O2
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Color White to off-white
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Sequence
Thr-Trp-Pro-Lys-His-Phe-Asp-Lys-His-Thr-Phe-Tyr-Ser-Ile-Leu-Lys-Leu-Gly-Lys-His
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Sequence Shortening
TWPKHFDKHTFYSILKLGKH
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Pureza y Documentación
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Ficha de datos (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Sakamoto K, et al., A novel LRP1-binding peptide L57 that crosses the blood brain barrier. Biochem Biophys Rep. 2017 Aug 12;12:135-139. [Content Brief]
[2]. Rodrigues JP, et al. Efficient LRP1-Mediated Uptake and Low Cytotoxicity of Peptide L57 In Vitro Shows Its Promise as CNS Drug Delivery Vector. J Pharm Sci. 2021;110(2):824-832. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
L57 acetate Related Classifications
HY-P10502A Related Classifications
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