L57
Based on 1 Customer Validation
L57 is a low-density lipoprotein receptor-related protein 1 (LRP1)-binding peptide. L57 exhibits high affinity for LRP1, with an EC50 of 45 nM. L57 possesses blood-brain barrier (BBB) permeability and plasma stability. L57 can serve as a carrier for central nervous system drug delivery.
For research use only. We do not sell to patients.
- Purity: 99.44%
- CAS No.: 2378836-93-0
- Formula: C120H175N31O27
- Molecular Weight:2483.87
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
L57 (EC50-determining concentrations; 1 h) selectively binds to recombinant human LRP1(CL4)-Fc with an EC50 of 45 nM in a plate ELISA assay[1].
Fluorescein-labeled L57 (3-30 μM; 4 h, 1 min) exhibits rapid, concentration-dependent, LRP1-mediated uptake in primary rat BMVECs and astrocytes, with significantly higher uptake efficiency than the reference peptide at matching concentrations, and shows limited uptake in LRP1-deficient PEA 10 cells[2].
Fluorescein-labeled L57 (3-30 μM; 4 h) shows excellent biocompatibility with no cytotoxicity observed in primary rat BMVECs or LRP1-deficient PEA 10 cells at concentrations up to 30 μM after 4 h of incubation[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Primary rat brain microvascular endothelial cells (BMVECs), LRP1-deficient PEA 10 mouse embryonic fibroblasts
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Concentration:3 μM, 10 μM, 30 μM
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Incubation Time:4 h
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Result:Resulted in high cell viability in both BMVECs and PEA 10 cells, with metabolic activity comparable to vehicle control conditions at all tested concentrations.
L57 (i.v.; single dose) results in modest brain delivery in male C57BL/6J mice, with a % input value of 0.042[1].
L57 demonstrates significant blood-brain barrier permeability and high LRP1 binding affinity in healthy mice, supporting its potential as a CNS drug delivery vector[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2378836-93-0
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Appearance Solid
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Molecular Weight 2483.87
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Formula C120H175N31O27
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Color White to off-white
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Sequence
Thr-Trp-Pro-Lys-His-Phe-Asp-Lys-His-Thr-Phe-Tyr-Ser-Ile-Leu-Lys-Leu-Gly-Lys-His
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Sequence Shortening
TWPKHFDKHTFYSILKLGKH
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 50 mg/mL (20.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Sakamoto K, et al. A novel LRP1-binding peptide L57 that crosses the blood brain barrier. Biochem Biophys Rep. 2017;12:135-139. Published 2017 Aug 12. [Content Brief]
[2]. Rodrigues JP, et al. Efficient LRP1-Mediated Uptake and Low Cytotoxicity of Peptide L57 In Vitro Shows Its Promise as CNS Drug Delivery Vector. J Pharm Sci. 2021;110(2):824-832. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.4026 mL | 2.0130 mL | 4.0260 mL | 10.0649 mL |
| 5 mM | 0.0805 mL | 0.4026 mL | 0.8052 mL | 2.0130 mL | |
| 10 mM | 0.0403 mL | 0.2013 mL | 0.4026 mL | 1.0065 mL | |
| 15 mM | 0.0268 mL | 0.1342 mL | 0.2684 mL | 0.6710 mL | |
| 20 mM | 0.0201 mL | 0.1006 mL | 0.2013 mL | 0.5032 mL |
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