2. Metabolic Enzyme/Protease
  3. LDLR
  4. L57

L57 is a low-density lipoprotein receptor-related protein 1 (LRP1)-binding peptide. L57 exhibits high affinity for LRP1, with an EC50 of 45 nM. L57 possesses blood-brain barrier (BBB) permeability and plasma stability. L57 can serve as a carrier for central nervous system drug delivery.

For research use only. We do not sell to patients.

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L57

L57 Chemical Structure

CAS No. : 2378836-93-0

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Based on 1 publication(s) in Google Scholar

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L57 Related Antibodies

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Description

L57 is a low-density lipoprotein receptor-related protein 1 (LRP1)-binding peptide. L57 exhibits high affinity for LRP1, with an EC50 of 45 nM. L57 possesses blood-brain barrier (BBB) permeability and plasma stability. L57 can serve as a carrier for central nervous system drug delivery[1].

In Vitro

L57 (EC50-determining concentrations; 1 h) selectively binds to recombinant human LRP1(CL4)-Fc with an EC50 of 45 nM in a plate ELISA assay[1].
Fluorescein-labeled L57 (3-30 μM; 4 h, 1 min) exhibits rapid, concentration-dependent, LRP1-mediated uptake in primary rat BMVECs and astrocytes, with significantly higher uptake efficiency than the reference peptide at matching concentrations, and shows limited uptake in LRP1-deficient PEA 10 cells[2].
Fluorescein-labeled L57 (3-30 μM; 4 h) shows excellent biocompatibility with no cytotoxicity observed in primary rat BMVECs or LRP1-deficient PEA 10 cells at concentrations up to 30 μM after 4 h of incubation[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

L57 Related Antibodies

Cell Cytotoxicity Assay[2]

Cell Line: Primary rat brain microvascular endothelial cells (BMVECs), LRP1-deficient PEA 10 mouse embryonic fibroblasts
Concentration: 3 μM, 10 μM, 30 μM
Incubation Time: 4 h
Result: Resulted in high cell viability in both BMVECs and PEA 10 cells, with metabolic activity comparable to vehicle control conditions at all tested concentrations.
In Vivo

L57 (in situ brain perfusion; 1.15 mL/min; 5 minutes) results in a significantly high blood-brain barrier permeability in male C57BL/6J mice, with a % input value of 0.220[1].
L57 (i.v.; single dose) results in modest brain delivery in male C57BL/6J mice, with a % input value of 0.042[1].
L57 demonstrates significant blood-brain barrier permeability and high LRP1 binding affinity in healthy mice, supporting its potential as a CNS drug delivery vector[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

2483.87

Formula

C120H175N31O27
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Thr-Trp-Pro-Lys-His-Phe-Asp-Lys-His-Thr-Phe-Tyr-Ser-Ile-Leu-Lys-Leu-Gly-Lys-His
Sequence Shortening

TWPKHFDKHTFYSILKLGKH
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (20.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.4026 mL 2.0130 mL 4.0260 mL
5 mM 0.0805 mL 0.4026 mL 0.8052 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.4026 mL 2.0130 mL 4.0260 mL 10.0649 mL
5 mM 0.0805 mL 0.4026 mL 0.8052 mL 2.0130 mL
10 mM 0.0403 mL 0.2013 mL 0.4026 mL 1.0065 mL
15 mM 0.0268 mL 0.1342 mL 0.2684 mL 0.6710 mL
20 mM 0.0201 mL 0.1006 mL 0.2013 mL 0.5032 mL
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L57 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L572378836-93-0L 57L-57LDLRLow-density lipoprotein receptorreceptor-mediated transcytosisblood-brain barrierLRP1 cluster 4LRP1-deficient cellshuman low-density lipoprotein receptor-related protein 1 cluster 4astrocytesbrain microvascular endothelial cellsprimary rat BMVECsmale C57BL/6J micePEA 10 cellsInhibitorinhibitorinhibit

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