2. Immunology/Inflammation
  3. TROP2
  4. hIMB1636

hIMB1636 is a humanized monoclonal antibody targeting Trop2. By binding to the conformational Trop2 epitope, hIMB1636 regulates related signaling pathways, triggers lysosomal endocytosis, and further induces apoptosis, cell cycle arrest, and antibody-dependent cellular cytotoxicity. hIMB1636 effectively inhibits tumor cell proliferation, migration and in vivo tumor growth, and also exerts bystander killing effect and mediates long-term retention. hIMB1636 can be conjugated with NOTA/DOTA for radiolabeling to enable immuno-PET imaging, or prepared as hIMB1636-LDP-AE to significantly inhibit the growth of breast cancer and lung cancer xenografts.

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hIMB1636

hIMB1636 Chemical Structure

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hIMB1636 Related Antibodies

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Description

hIMB1636 is a humanized monoclonal antibody targeting Trop2. By binding to the conformational Trop2 epitope, hIMB1636 regulates related signaling pathways, triggers lysosomal endocytosis, and further induces apoptosis, cell cycle arrest, and antibody-dependent cellular cytotoxicity. hIMB1636 effectively inhibits tumor cell proliferation, migration and in vivo tumor growth, and also exerts bystander killing effect and mediates long-term retention. hIMB1636 can be conjugated with NOTA/DOTA for radiolabeling to enable immuno-PET imaging, or prepared as hIMB1636-LDP-AE to significantly inhibit the growth of breast cancer and lung cancer xenografts[1][2][3][4].

Species Reactivity

Human
In Vitro

hIMB1636 (1.56-50 nM) binds to recombinant human Trop2 protein with a Kd of 0.603 nM[1].
hIMB1636 (5-10 μM; 72 h) potently inhibits proliferation of MDA-MB-468 and MDA-MB-231 cells in a Trop2-dependent manner, with superior activity compared to sacituzumab[2].
hIMB1636 (5-10 μM; 36 h (MDA-MB-468); 12 h (MDA-MB-231)) inhibits migration of MDA-MB-468 and MDA-MB-231 cells in a Trop2-dependent manner[2].
hIMB1636 (5 μM; 24 h) induces caspase-dependent apoptosis in MDA-MB-468 and MDA-MB-231 cells by upregulating cleaved caspase-9, cleaved caspase-3, and cleaved PARP[2].
hIMB1636 (5 μM; 24 h) significantly increases the proportion of apoptotic cells in MDA-MB-468 and MDA-MB-231 cells[2].
hIMB1636 binds specifically to T3M-4 pancreatic cancer cells, which show the highest Trop2 expression among tested pancreatic cancer cell lines, as confirmed by multiple cell-based binding assays[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

hIMB1636 Related Antibodies

Cell Proliferation Assay[2]

Cell Line: MDA-MB-468, MDA-MB-231
Concentration: 5 μM, 10 μM
Incubation Time: 72 h
Result: Inhibited cell proliferation in a dose-dependent manner in MDA-MB-468 cells, with 10 μM hIMB1636 reducing proliferation to 22.60% of control levels.
Reduced proliferation to ~15% of control levels in MDA-MB-231 cells at both 5 μM and 10 μM, indicating saturation of surface Trop2 at the lower concentration.
Exhibited significantly stronger proliferation inhibition than sacituzumab at matching concentrations in both cell lines.

Western Blot Analysis[2]

Cell Line: MDA-MB-468, MDA-MB-231
Concentration: 5 μM
Incubation Time: 24 h
Result: Reduced expression of cyclin D1 and CDK6, and increased expression of P21, compared to control cells.\nUpregulated expression of cleaved caspase-9, cleaved caspase-3, and cleaved PARP, indicating activation of the caspase-dependent apoptotic pathway.

Apoptosis Analysis[2]

Cell Line: MDA-MB-468, MDA-MB-231
Concentration: 5 μM
Incubation Time: 24 h
Result: Significantly increased the ratio of early and late apoptotic cells in both cell lines, with MDA-MB-468 cells showing an increase from 8.25% to 12.89% apoptotic cells, and MDA-MB-231 cells showing an increase from 3.70% to 11.05% apoptotic cells.
In Vivo

hIMB1636 (0.8 mg/kg; i.v.; every five days; 4 total injections) exhibits no antitumor efficacy in HCC827 lung cancer xenografts, with tumor growth matching the control group and no significant body weight loss[1].
hIMB1636 (25-50 mg/kg; i.v.; every 5 days; 4 total doses) exhibits potent in vivo antitumor activity in MDA-MB-468 xenografts, achieving tumor inhibition rates of 61.4% at 50 mg/kg and 58.2% at 25 mg/kg[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c nude (6-week-old female; HCC827 cells injected subcutaneously into right flank)[1]
Dosage: 0.8 mg/kg
Administration: i.v.; every five days; 4 total injections
Result: Showed no antitumor effects, with tumor volumes similar to that of the control group.
Caused no significant body weight loss.
Animal Model: BALB/c nude (6-week-old female)[2]
Dosage: 50 mg/kg; 25 mg/kg
Administration: i.v.; every 5 days; 4 total doses
Result: Produced a tumor inhibition rate of 61.4% at 50 mg/kg on day 37 post-tumor implantation.
Produced a tumor inhibition rate of 58.2% at 25 mg/kg on day 37 post-tumor implantation.
Caused no significant weight loss.
Gene ID

4070  [NCBI]

Accession
Target

TROP2
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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hIMB1636 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

hIMB1636hIMB 1636hIMB-1636TROP2EGP-1M1S1GA733-1stomach cancerHCC827colon cancerpancreatic cancerTrop2breast cancerlung cancertriple-negative breast cancerovarian cancerlung squamous carcinomaInhibitorinhibitorinhibit

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