Pac-525
Pac-525 is a tryptophan-rich cationic antibacterial peptide and membrane-disrupting agent. Pac-525 exhibits antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria, anaerobic bacteria, *Candida albicans* and *Fusarium solani*. Pac-525 interacts strongly with negatively charged phospholipid vesicles, induces vesicle dye release and disrupts microbial membranes. Pac-525 shows cytotoxicity against macrophages. Pac-525 can be used in studies related to bacterial and fungal infections.
For research use only. We do not sell to patients.
- CAS No.: 678967-50-5
- Formula: C68H100N22O10
- Molecular Weight:1385.66
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Pac-525 (0.98-500 µg/mL; 24 h) potently inhibits the growth of planktonic *Escherichia coli* KV203, with an MIC of 62.5 µg/mL and an MIC50 of 41.0 µg/mL[1].
Pac-525 (2-4 μM; 18 h) exhibits antibacterial activity against E. coli (ATCC 25922, ATCC 10536), Pseudomonas aeruginosa (ATCC 27853), and S. aureus (ATCC 29213, ATCC 33591)[2].
Pac-525 (1.95-500 µg/mL; 24 h) exhibits cytotoxicity against RAW 264.7 cells, with an IC70 of 330.88 µg/mL, and reduces cell viability to 40% at a concentration of 500 µg/mL[1].
Pac-525 (350 μM; 1 h) exhibits extremely low hemolytic activity against human red blood cells, and can induce 50% cell lysis[2].
Pac-525 (~1 μM, 25°C) exhibits strong membrane-disrupting activity and induces approximately 100% calcein release from large unilamellar vesicles (LUVs) composed of POPG, POPC/POPG (3:1), and POPC[2].
Pac-525 (250 µg/mL; 4 h) reduces the intracellular load of *E. coli* KV203 by 23.6% in infected RAW 264.7 cells, but exhibits very limited penetration ability into RAW 264.7 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RAW 264.7 murine macrophage cells
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Concentration:1.95, 3.91, 7.8, 15.63, 31.25, 62.5, 125, 250, 500 µg/mL
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Incubation Time:24 h
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Result:Exhibited an IC70 (concentration reducing cell viability by 30%) of 330.88 µg/mL on RAW 264.7 cells.
Reduced cell viability to 40% at the highest tested concentration of 500 µg/mL.
Chemical Information
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CAS No. 678967-50-5
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Molecular Weight 1385.66
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Formula C68H100N22O10
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Sequence
Lys-Trp-Arg-Arg-Trp-Val-Arg-Trp-Ile
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Sequence Shortening
KWRRWVRWI
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Coufalova M, et al. Antibacterial activity of the novel peptide Pac-525 with the RGD motif against intracellular Escherichia coli. Scientific reports. 2025 Jun 06;15(1):19995. [Content Brief]
[2]. Wei SY, et al. Solution structure of a novel tryptophan-rich peptide with bidirectional antimicrobial activity. Journal of bacteriology. 2006 Jan;188(1):328-34. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pac-525 Related Classifications
HY-P11907 Related Classifications
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- Pac-525
- 678967-50-5
- Pac525
- Pac 525
- Bacterial
- Fungal
- Fusarium solani
- Candida albicans
- gram-positive bacteria
- adherent-invasive Escherichia coli KV203
- negatively charged phospholipid vesicles
- RAW 264.7 murine macrophage cells
- anaerobic bacteria
- human red blood cells
- macrophage cells
- gram-negative bacteria
- Inhibitor
- inhibitor
- inhibit