CNQX disodium
Based on 16 publication(s) in Google Scholar
CNQX disodium (FG9065 disodium) is a potent and competitive AMPA/kainate receptor antagonist with IC50s of 0.3 μM and 1.5 μM, respectively. CNQX disodium is a competitive non-NMDA receptor antagonist. CNQX disodium blocks the expression of fear-potentiated startle in rats.
For research use only. We do not sell to patients.
- Purity: 99.95%
- CAS No.: 479347-85-8
- Formula: C9H2N4Na2O4
- Molecular Weight:276.12
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) CNQX disodium
More- Nat Neurosci. 2023 May;26(5):751-764. [Abstract]
- Nat Commun. 2025 May 16;16(1):4560. [Abstract]
- Nat Commun. 2024 May 15;15(1):4122. [Abstract]
- Environ Sci Technol. 2023 Aug 22;57(33):12222-12233. [Abstract]
- Cell Death Dis. 2022 Sep 12;13(9):786. [Abstract]
- Acta Biomater. 2022 Oct 15:152:255-272. [Abstract]
- Biomed Pharmacother. 2022 Jan:145:112446. [Abstract]
- PLoS Biol. 2025 Jan 27;23(1):e3003011. [Abstract]
- Cell Rep. 2025 May 14;44(5):115712. [Abstract]
- Commun Biol. 2025 Dec 24;9(1):39. [Abstract]
- J Cell Mol Med. 2021 Aug;25(15):7342-7353. [Abstract]
- J Neurosci. 2024 Apr 10;44(15):e2062232024. [Abstract]
- Front Cell Neurosci. 2019 Jun 25:13:276. [Abstract]
- Cereb Cortex. 2024 Oct 3;34(10):bhae399. [Abstract]
- J Integr Neurosci. 2022 Jun 28;21(4):121. [Abstract]
- SSRN. 2025 Jun 18.
Customer Validation & Images
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
All iGluR Isoforms
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Biological Activity
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Kainate Receptor |
CNQX disodium (FG9065 disodium; 2-5 μM) reversibly blocks the Schaffer collateral and mossy fibre excitatory postsynaptic potential (EPSP), while sparing the fast and slow GABA-mediated inhibition in superfusion of hippocampal slices[2].
CNQX disodium (1-5 μM) produces a selective and dose-dependent reduction in the amplitude of the monosynaptic component of the DR-VRR recorded from lumbar spinal segments[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
The bilateral infusion of CNQX disodium (0.5 or 1.25 μg) into the amygdala or dorsal hippocampus 10 min prior to a retention test partially blocks the expression of stepdown inhibitory avoidance in rats 24 h after training. CNQX disodium causes a complete blockade at a dose of 0.5 μg[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Wistar rats weighing 180-200 g[4]
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Dosage:0.75, 1.5, and 3 mg/kg
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Administration:IP; 20 min before testing
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Result:Decreased the number of cocaine (IV; 0.25 mg/infusion) responses in a dose-dependent manner during the first 15-min cocaine-free interval.
Chemical Information
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CAS No. 479347-85-8
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Appearance Solid
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Molecular Weight 276.12
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Formula C9H2N4Na2O4
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Color Brown to red
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SMILES
N#CC1=CC2=C([N-]C(C([N-]2)=O)=O)C=C1[N+]([O-])=O.[Na+].[Na+]
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Synonyms
FG9065 disodium
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (16)
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Journal Impact Factor
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Most Recent
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Nat Neurosci
Emergence of consciousness from anesthesia through ubiquitin degradation of KCC2 in the ventral posteromedial nucleus of the thalamus. [Abstract]2023 May;26(5):751-764. PMID: 36973513 -
Nat Commun
A ventral pallidum-locus coeruleus-lateral hypothalamus pathway modulates brain arousal in freely behaving and isoflurane-anesthetized male mice. [Abstract]2025 May 16;16(1):4560. PMID: 40379709 -
Nat Commun
2024 May 15;15(1):4122. PMID: 38750027
CNQX disodium purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 May 15;15(1):4122. [Abstract]
Light-evoked EPSCs in vCE L5a PCs were abolished after bath application of tetrodotoxin (TTX) but were then reintroduced following application of 4-Aminopyridine, indicating the monosynaptic nature of connections from V2 to vCE L5a. These restored responses were blocked by AMPA receptor antagonist CNQX.
CNQX disodium purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 May 15;15(1):4122. [Abstract]
The light-evoked EPSCs were abolished by TTX and rescued by 4-AP, but severely attenuated by CNQX.
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Environ Sci Technol
Chronic Pb Exposure Induces Anxiety and Depression-like Behaviors in Mice via Excitatory Neuronal Hyperexcitability in Ventral Hippocampal Dentate Gyrus. [Abstract]2023 Aug 22;57(33):12222-12233. PMID: 37559393 -
Cell Death Dis
Crosstalk of Synapsin1 palmitoylation and phosphorylation controls the dynamicity of synaptic vesicles in neurons. [Abstract]2022 Sep 12;13(9):786. PMID: 36097267 -
Acta Biomater
2022 Oct 15:152:255-272. PMID: 36041647 -
Biomed Pharmacother
Cordycepin suppresses glutamatergic and GABAergic synaptic transmission through activation of A1 adenosine receptor in rat hippocampal CA1 pyramidal neurons. [Abstract]2022 Jan:145:112446. PMID: 34808556 -
PLoS Biol
Supraspinal facilitation of painful stimuli by glutamatergic innervation from the retrosplenial to the anterior cingulate cortex. [Abstract]2025 Jan 27;23(1):e3003011. PMID: 39869643
CNQX disodium purchased from MedChemExpress. Usage Cited in: PLoS Biol. 2025 Jan 27;23(1):e3003011. [Abstract]
Sample traces of eEPSCs in the RSC-ACC projections with the perfusion of D-AP5 (50 μM), GYKI53655 (100 μM), and CNQX (20 μM) sequentially under the presence of picrotoxin (50 μM) (one-way RM ANOVA with Sidak multiple comparisons tests.
CNQX disodium purchased from MedChemExpress. Usage Cited in: PLoS Biol. 2025 Jan 27;23(1):e3003011. [Abstract]
Sample amplitude curve of eEPSCs in the RSC-ACC projections with the perfusion of D-AP5 (50 μM), GYKI53655 (100 μM), and CNQX (20 μM) sequentially under the presence of picrotoxin (50 μM) (one-way RM ANOVA with Sidak multiple comparisons tests.
CNQX disodium purchased from MedChemExpress. Usage Cited in: PLoS Biol. 2025 Jan 27;23(1):e3003011. [Abstract]
Averaged amplitude of eEPSCs in the RSC-ACC projections with the perfusion of D-AP5 (50 μM), GYKI53655 (100 μM), and CNQX (20 μM) sequentially under the presence of picrotoxin (50 μM) (one-way RM ANOVA with Sidak multiple comparisons tests.
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Cell Rep
Septo-subicular cholinergic circuit promotes seizure development via astrocytic inflammation. [Abstract]2025 May 14;44(5):115712. PMID: 40372911 -
Commun Biol
Neural mechanisms of the V2M-PrL pathway mediate blue light-induced spatial memory impairment. [Abstract]2025 Dec 24;9(1):39. PMID: 41444415 -
J Cell Mol Med
The molecular mechanism underlying mitophagy-mediated hippocampal neuron apoptosis in diabetes-related depression. [Abstract]2021 Aug;25(15):7342-7353. PMID: 34213839 -
J Neurosci
Insula-amygdala and insula-thalamus pathways are involved in comorbid chronic pain and depression-like behavior in mice. [Abstract]2024 Apr 10;44(15):e2062232024. PMID: 38453468 -
Front Cell Neurosci
Pin1 Is Regulated by CaMKII Activation in Glutamate-Induced Retinal Neuronal Regulated Necrosis. [Abstract]2019 Jun 25:13:276. PMID: 31293391 -
Cereb Cortex
A circuit from lateral hypothalamic to dorsal hippocampal dentate gyrus modulates behavioral despair in mice. [Abstract]2024 Oct 3;34(10):bhae399. PMID: 39367727 -
J Integr Neurosci
2022 Jun 28;21(4):121. PMID: 35864772 -
Solvent & Solubility
DMSO : 10.53 mg/mL (38.14 mM; ultrasonic and warming and adjust pH to 2 with HCl and heat to 70°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Purity & Documentation
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Data Sheet (281 KB)
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SDS (761 KB)
- English - EN (761 KB)
- Français - FR (761 KB)
- Deutsch - DE (761 KB)
- Norwegian - NO (761 KB)
- Español - ES (761 KB)
- Swedish - SV (761 KB)
- Italian - IT (761 KB)
- Portuguese - PT (761 KB)
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Handling Instructions (2659 KB)
References
[1]. T Honoré, et al. Quinoxalinediones: Potent Competitive non-NMDA Glutamate Receptor Antagonists. Science. 1988 Aug 5;241(4866):701-3. [Content Brief]
[2]. Neuman RS, et al. Blockade of excitatory synaptic transmission by 6-cyano-7-nitroquinoxaline-2,3-dione(CNQX) in the hippocampus in vitro. Neurosci Lett. 1988 Sep 23;92(1):64-8. [Content Brief]
[3]. Alford S, et al. CNQX and DNQX block non-NMDA synaptic transmission but not NMDA-evoked locomotion in lamprey spinal cord. Brain Res. 1990 Jan 8;506(2):297-302. [Content Brief]
[4]. Pia Bäckström, et al. Attenuation of Cocaine-Seeking Behaviour by the AMPA/kainate Receptor Antagonist CNQX in Rats. Psychopharmacology (Berl). 2003 Feb;166(1):69-76. [Content Brief]
[5]. Kim M, et al. Infusion of the non-NMDA receptor antagonist CNQX into the amygdala blocks the expression of fear-potentiated startle. Behav Neural Biol. 1993 Jan;59(1):5-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.6216 mL | 18.1081 mL | 36.2161 mL | 90.5403 mL |
| 5 mM | 0.7243 mL | 3.6216 mL | 7.2432 mL | 18.1081 mL | |
| 10 mM | 0.3622 mL | 1.8108 mL | 3.6216 mL | 9.0540 mL | |
| 15 mM | 0.2414 mL | 1.2072 mL | 2.4144 mL | 6.0360 mL | |
| 20 mM | 0.1811 mL | 0.9054 mL | 1.8108 mL | 4.5270 mL | |
| 25 mM | 0.1449 mL | 0.7243 mL | 1.4486 mL | 3.6216 mL | |
| 30 mM | 0.1207 mL | 0.6036 mL | 1.2072 mL | 3.0180 mL |
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