Mitonafide
Based on 3 publication(s) in Google Scholar
Mitonafide (NSC 300288) is a cytostatic agent. Mitonafide binds to double-stranded DNA through intercalation, and inhibits DNA and RNA synthesis. Mitonafide is an antitumor agent that can be used in the research of cancers, such as non-small cell lung cancer (NSCLC), leukemia.
For research use only. We do not sell to patients.
- Purity: 99.73%
- CAS No.: 54824-17-8
- Formula: C16H15N3O4
- Molecular Weight:313.31
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
Publications Citing Use of MedChemExpress (MCE) Mitonafide
MoreAll DNA/RNA Synthesis Isoforms
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Biological Activity
DNA and RNA synthesis[1]
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| A2780 | IC50 |
5.22 μM
Compound: Mitonafide
|
Antiproliferative activity against human A2780 cells assessed as cell growth inhibition by MTT assay
Antiproliferative activity against human A2780 cells assessed as cell growth inhibition by MTT assay
|
[PMID: 36894107] |
| A-375 | IC50 |
5.3 μM
Compound: B1
|
Cytotoxicity against human A375 cells in hypoxic condition after 24 hrs by MTT assay
Cytotoxicity against human A375 cells in hypoxic condition after 24 hrs by MTT assay
|
[PMID: 17331719] |
| A549 | GI50 |
65 nM
Compound: Mitonafide
|
Concentration required to inhibit human small cell lung cancer A549 cell growth by 50% after 120 hours; determined by MTT assay
Concentration required to inhibit human small cell lung cancer A549 cell growth by 50% after 120 hours; determined by MTT assay
|
[PMID: 15974599] |
| A549 | IC50 |
0.012 μM
Compound: Mitonafide
|
Antiproliferative activity against human A549 cells by MTT assay
Antiproliferative activity against human A549 cells by MTT assay
|
[PMID: 33109400] |
| A549 | IC50 |
1.5 μM
Compound: MAF
|
Antiproliferative activity against human A549 cells assessed as inhibition of cell growth
Antiproliferative activity against human A549 cells assessed as inhibition of cell growth
|
[PMID: 37974948] |
| A549 | IC50 |
2.4 μM
Compound: 3
|
Antiproliferative activity against A549 cells after 72 hrs by MTT assay
Antiproliferative activity against A549 cells after 72 hrs by MTT assay
|
[PMID: 17658777] |
| A549 | IC50 |
3.6 μM
Compound: Mitonafide
|
Antiproliferative activity against human A549 cells incubated for 48 hrs by MTT assay
Antiproliferative activity against human A549 cells incubated for 48 hrs by MTT assay
|
[PMID: 38784471] |
| A549 | IC50 |
7.94 μM
Compound: Mitonafide
|
Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
|
[PMID: 32111436] |
| CHL-1 | IC50 |
0.5 μM
Compound: Mitonafide
|
Antiproliferative activity against human CHL-1 cells harboring wild type BRAF after 72 hrs by MTT assay
Antiproliferative activity against human CHL-1 cells harboring wild type BRAF after 72 hrs by MTT assay
|
[PMID: 28458134] |
| CHL-1 | IC50 |
0.9 μM
Compound: Mitonafide
|
Antiproliferative activity against human CHL-1 cells harboring wild type BRAF after 48 hrs by MTT assay
Antiproliferative activity against human CHL-1 cells harboring wild type BRAF after 48 hrs by MTT assay
|
[PMID: 28458134] |
| CHL-1 | IC50 |
2.8 μM
Compound: Mitonafide
|
Antiproliferative activity against human CHL-1 cells harboring wild type BRAF after 24 hrs by MTT assay
Antiproliferative activity against human CHL-1 cells harboring wild type BRAF after 24 hrs by MTT assay
|
[PMID: 28458134] |
| CNE-2 | IC50 |
3.42 μM
Compound: Mitonafide
|
Antiproliferative activity against human CNE-2 cells assessed as cell growth inhibition by MTT assay
Antiproliferative activity against human CNE-2 cells assessed as cell growth inhibition by MTT assay
|
[PMID: 36894107] |
| HCT-116 | GI50 |
250 nM
Compound: Mitonafide
|
Concentration required to inhibit the growth of human colon cancer HCT116 cell line by 50% to initial after 120 hours; determined using MTT assay
Concentration required to inhibit the growth of human colon cancer HCT116 cell line by 50% to initial after 120 hours; determined using MTT assay
|
[PMID: 15974599] |
| HCT-116 | IC50 |
12.23 μM
Compound: Mitonafide
|
Antiproliferative activity against human HCT-116 cells incubated for 48 hrs by MTT assay
Antiproliferative activity against human HCT-116 cells incubated for 48 hrs by MTT assay
|
[PMID: 38784471] |
| HCT-15 | IC50 |
2.6 μM
Compound: 3
|
Antiproliferative activity against HCT15 cells after 72 hrs by MTT assay
Antiproliferative activity against HCT15 cells after 72 hrs by MTT assay
|
[PMID: 17658777] |
| HepG2 | IC50 |
0.012 μM
Compound: Mitonafide
|
Antiproliferative activity against human HepG2 cells incubated for 48 hrs by MTT assay
Antiproliferative activity against human HepG2 cells incubated for 48 hrs by MTT assay
|
[PMID: 37972528] |
| HepG2 | IC50 |
10.88 μM
Compound: Mitonafide
|
Antiproliferative activity against human HepG2 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human HepG2 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
|
[PMID: 32111436] |
| HK-2 | IC50 |
2.13 μM
Compound: Mitonafide
|
Cytotoxicity against HK-2 cells incubated for 48 hrs by MTT assay
Cytotoxicity against HK-2 cells incubated for 48 hrs by MTT assay
|
[PMID: 38784471] |
| HL-60 | GI50 |
45 nM
Compound: Mitonafide
|
Concentration required to inhibit the growth of human leukemia cancer HL-60 cell line by 50% after 120 hours; determined by MTT assay
Concentration required to inhibit the growth of human leukemia cancer HL-60 cell line by 50% after 120 hours; determined by MTT assay
|
[PMID: 15974599] |
| HL-60 | IC50 |
1.6 μM
Compound: Mitonafide
|
Cytotoxicity against human HL60 cells after 24 hrs by MTT assay
Cytotoxicity against human HL60 cells after 24 hrs by MTT assay
|
[PMID: 19954251] |
| Hs 683 | IC50 |
3.7 μM
Compound: 3
|
Antiproliferative activity against Hs683 cells after 72 hrs by MTT assay
Antiproliferative activity against Hs683 cells after 72 hrs by MTT assay
|
[PMID: 17658777] |
| HT-29 | IC50 |
0.22 μM
Compound: 4a
|
Cytotoxicity against human HT-29 cells after 144 hrs
Cytotoxicity against human HT-29 cells after 144 hrs
|
[PMID: 18774722] |
| HT-29 | IC50 |
0.6 μM
Compound: Mitonafide
|
In vitro cytotoxicity against HT-29 (human colon adenocarcinoma) cell line.
In vitro cytotoxicity against HT-29 (human colon adenocarcinoma) cell line.
|
[PMID: 9046334] |
| HUVEC | IC50 |
5.9 μM
Compound: Mitonafide
|
Cytotoxicity against HUVEC cells incubated for 48 hrs by MTT assay
Cytotoxicity against HUVEC cells incubated for 48 hrs by MTT assay
|
[PMID: 38784471] |
| L02 | IC50 |
8.51 μM
Compound: Mitonafide
|
Cytotoxicity against human HL7702 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Cytotoxicity against human HL7702 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
|
[PMID: 32111436] |
| LoVo | IC50 |
0.4 μM
Compound: 3
|
Antiproliferative activity against LoVo cells after 72 hrs by MTT assay
Antiproliferative activity against LoVo cells after 72 hrs by MTT assay
|
[PMID: 17658777] |
| MCF7 | GI50 |
245 nM
Compound: Mitonafide
|
Concentration required to inhibit the growth of human breast cancer MCF-7 cell line by 50% after 120 hours; determined by MTT assay
Concentration required to inhibit the growth of human breast cancer MCF-7 cell line by 50% after 120 hours; determined by MTT assay
|
[PMID: 15974599] |
| MDA-MB-231 | IC50 |
7.59 μM
Compound: Mitonafide
|
Antiproliferative activity against human MDA-MB-231 cells incubated for 48 hrs by MTT assay
Antiproliferative activity against human MDA-MB-231 cells incubated for 48 hrs by MTT assay
|
[PMID: 38784471] |
| MGC-803 | IC50 |
3.32 μM
Compound: Mitonafide
|
Antiproliferative activity against human MGC-803 cells assessed as cell growth inhibition by MTT assay
Antiproliferative activity against human MGC-803 cells assessed as cell growth inhibition by MTT assay
|
[PMID: 36894107] |
| MGC-803 | IC50 |
6.8 μM
Compound: Mitonafide
|
Antiproliferative activity against human MGC-803 cells by MTT assay
Antiproliferative activity against human MGC-803 cells by MTT assay
|
[PMID: 33109400] |
| MGC-803 | IC50 |
6.81 μM
Compound: Mitonafide
|
Antiproliferative activity against human MGC-803 cells incubated for 48 hrs by MTT assay
Antiproliferative activity against human MGC-803 cells incubated for 48 hrs by MTT assay
|
[PMID: 37972528] |
| MGC-803 | IC50 |
6.81 μM
Compound: Mitonafide
|
Antiproliferative activity against human MGC-803 cells incubated for 48 hrs by MTT assay
Antiproliferative activity against human MGC-803 cells incubated for 48 hrs by MTT assay
|
[PMID: 38784471] |
| NB-4 | IC50 |
3.4 μM
Compound: MAF
|
Antiproliferative activity against human NB4 cells assessed as inhibition of cell growth
Antiproliferative activity against human NB4 cells assessed as inhibition of cell growth
|
[PMID: 37974948] |
| PC-3 | GI50 |
0.15 μM
Compound: MIT
|
Cytotoxicity against PC3 cells by SRB assay after 48 hrs
Cytotoxicity against PC3 cells by SRB assay after 48 hrs
|
[PMID: 17125272] |
| SK-MEL-2 | GI50 |
150 nM
Compound: Mitonafide
|
Concentration required to inhibit the growth of human malenoma cancer SK-MEL-2 cell line by 50% after 120 hours; determined by MTT assay
Concentration required to inhibit the growth of human malenoma cancer SK-MEL-2 cell line by 50% after 120 hours; determined by MTT assay
|
[PMID: 15974599] |
| SK-OV-3 | IC50 |
1.38 μM
Compound: Mitonafide
|
Antiproliferative activity against human SK-OV-3 cells by MTT assay
Antiproliferative activity against human SK-OV-3 cells by MTT assay
|
[PMID: 33109400] |
| SK-OV-3 | IC50 |
1.38 μM
Compound: Mitonafide
|
Antiproliferative activity against human SK-OV-3 cells incubated for 48 hrs by MTT assay
Antiproliferative activity against human SK-OV-3 cells incubated for 48 hrs by MTT assay
|
[PMID: 37972528] |
| SK-OV-3 | IC50 |
6.26 μM
Compound: Mitonafide
|
Antiproliferative activity against human SKOV3 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human SKOV3 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
|
[PMID: 32111436] |
| SMMC-7721 | IC50 |
3.22 μM
Compound: Mitonafide
|
Antiproliferative activity against human SMMC-7721 cells by MTT assay
Antiproliferative activity against human SMMC-7721 cells by MTT assay
|
[PMID: 33109400] |
| SMMC-7721 | IC50 |
6.94 μM
Compound: Mitonafide
|
Antiproliferative activity against human SMMC7721 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human SMMC7721 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
|
[PMID: 32111436] |
| T-24 | IC50 |
0.012 μM
Compound: Mitonafide
|
Antiproliferative activity against human T24 cells incubated for 48 hrs by MTT assay
Antiproliferative activity against human T24 cells incubated for 48 hrs by MTT assay
|
[PMID: 38784471] |
| T-24 | IC50 |
1.11 μM
Compound: Mitonafide
|
Antiproliferative activity against human T-24 cells by MTT assay
Antiproliferative activity against human T-24 cells by MTT assay
|
[PMID: 33109400] |
| T-24 | IC50 |
1.11 μM
Compound: Mitonafide
|
Antiproliferative activity against human T24 cells incubated for 48 hrs by MTT assay
Antiproliferative activity against human T24 cells incubated for 48 hrs by MTT assay
|
[PMID: 37972528] |
| T-24 | IC50 |
3.25 μM
Compound: Mitonafide
|
Antiproliferative activity against human T24 cells assessed as cell growth inhibition by MTT assay
Antiproliferative activity against human T24 cells assessed as cell growth inhibition by MTT assay
|
[PMID: 36894107] |
| T-24 | IC50 |
5.01 μM
Compound: Mitonafide
|
Antiproliferative activity against human T24 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Antiproliferative activity against human T24 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
|
[PMID: 32111436] |
| U-251 | IC50 |
9.68 μM
Compound: Mitonafide
|
Antiproliferative activity against human U-251 cells incubated for 48 hrs by MTT assay
Antiproliferative activity against human U-251 cells incubated for 48 hrs by MTT assay
|
[PMID: 38784471] |
| U-373MG ATCC | IC50 |
1.3 μM
Compound: 3
|
Antiproliferative activity against U373MG cells after 72 hrs by MTT assay
Antiproliferative activity against U373MG cells after 72 hrs by MTT assay
|
[PMID: 17658777] |
| UACC-903 | IC50 |
0.5 μM
Compound: Mitonafide
|
Antiproliferative activity against human UACC-903 cells harboring BRAF V600E mutant after 72 hrs by MTT assay
Antiproliferative activity against human UACC-903 cells harboring BRAF V600E mutant after 72 hrs by MTT assay
|
[PMID: 28458134] |
| UACC-903 | IC50 |
0.8 μM
Compound: Mitonafide
|
Antiproliferative activity against human UACC-903 cells harboring BRAF V600E mutant after 48 hrs by MTT assay
Antiproliferative activity against human UACC-903 cells harboring BRAF V600E mutant after 48 hrs by MTT assay
|
[PMID: 28458134] |
| UACC-903 | IC50 |
11.4 μM
Compound: Mitonafide
|
Antiproliferative activity against human UACC-903 cells harboring BRAF V600E mutant after 24 hrs by MTT assay
Antiproliferative activity against human UACC-903 cells harboring BRAF V600E mutant after 24 hrs by MTT assay
|
[PMID: 28458134] |
| V79 | IC50 |
41.6 μM
Compound: B1
|
Cytotoxicity against V79 cells in hypoxic condition after 24 hrs by MTT assay
Cytotoxicity against V79 cells in hypoxic condition after 24 hrs by MTT assay
|
[PMID: 17331719] |
Mitonafide inhibits DNA and RNA synthesis and induces single-strand breaks in the DNA of chinese hamster ovary cells[1].
The incubation of Mitonafide with rat liver microsomes and NADPH under anaerobic conditions results in the formation of a metabolite identified as 5-aminomitonafide[2].
Mitonafide (25, 50 μM, 1 h) induces single-stand breaks in the DNA of L1210 cells[2].
Mitonafide (10-100 μM) exhibits cytotoxic effect in the HOP-62 lung cell line[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:SK-OV-3, HepG2, A-549, T-24, SMMC-7721, HL-7702
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Concentration:0-100 μM respectively.
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Incubation Time:48 h
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Result:Inhibited cell viability with IC50 values of 6.26, 10.88, 7.94, 5.01, 6.94, 8.51 μM.
Mitonafide (5 mg/kg, i.p., twice a day) shows anticancer activity in HepG2 xenograft model[6].
Mitonafide (single i.p. injection, S-180 bearing mice) shows the LD50 value of 10.0 mg/kg[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:S-180 bearing mice[4]
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Dosage:0.5 mg/kg and 1 mg/kg
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Administration:Intraperitoneal injection (i.p.) for 1-7 days
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Result:Increased in median survival times.
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Animal Model:HepG2 xenograft model [6]
-
Dosage:5 mg/kg
-
Administration:Intraperitoneal injection (i.p.), twice a day.
-
Result:Exhibited a relative tumor increment rates (T/C) value of 28.8%.
Chemical Information
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CAS No. 54824-17-8
-
Appearance Solid
-
Molecular Weight 313.31
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Formula C16H15N3O4
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Color Light yellow to yellow
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SMILES
O=C1N(C(C2=CC([N+]([O-])=O)=CC3=CC=CC1=C23)=O)CCN(C)C
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Synonyms
NSC 300288
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (3)
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Journal Impact Factor
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Most Recent
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Pharmaceutics
Benzofuran-Annulated Naphthalimides Trigger Replication Stress, DNA Damage, and p53-Dependent Cell Cycle Arrest. [Abstract]2026 Jun 20;18(6):754. PMID: 42357370 -
Pharmaceutics
Benzodioxin-Annulated Naphthalimides as Potent DNA Replication Stress Inducers with Dual p53-Dependent and Independent Antitumor Activity. [Abstract]2026 Jan 27;18(2):167. PMID: 41754909 -
Solvent & Solubility
DMSO : 62.5 mg/mL (199.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (280 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Llombart M, et al. Phase I study of mitonafide in solid tumors. Invest New Drugs. 1992 Aug;10(3):177-81. [Content Brief]
[2]. inha BK, et al. Mechanism of DNA strand breaks by mitonafide, an imide derivative of 3-nitro-1,8-naphthalic acid. Biochem Pharmacol. 1985 Nov 1;34(21):3845-52. [Content Brief]
[3]. Rosell R, et al. Phase I study of mitonafide in 120 hour continuous infusion in non-small cell lung cancer. Invest New Drugs. 1992 Aug;10(3):171-5. [Content Brief]
[4]. Samanta S, et al. Antitumor activity of Nitronaphthal-NU, a novel mixed-function agent. J Exp Ther Oncol. 2005;5(1):15-22. [Content Brief]
[5]. Pain A, et al. Evaluation of naphthalmustine, a nitrogen mustard derivative of naphthalimide as a rationally-designed anticancer agent. J Exp Clin Cancer Res. 2003 Sep;22(3):411-8. [Content Brief]
[6]. Xin M, et al. Design, synthesis and biological evaluation of 3-nitro-1,8-naphthalimides as potential antitumor agents. Bioorg Med Chem Lett. 2020 Apr 15;30(8):127051. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1917 mL | 15.9586 mL | 31.9173 mL | 79.7932 mL |
| 5 mM | 0.6383 mL | 3.1917 mL | 6.3835 mL | 15.9586 mL | |
| 10 mM | 0.3192 mL | 1.5959 mL | 3.1917 mL | 7.9793 mL | |
| 15 mM | 0.2128 mL | 1.0639 mL | 2.1278 mL | 5.3195 mL | |
| 20 mM | 0.1596 mL | 0.7979 mL | 1.5959 mL | 3.9897 mL | |
| 25 mM | 0.1277 mL | 0.6383 mL | 1.2767 mL | 3.1917 mL | |
| 30 mM | 0.1064 mL | 0.5320 mL | 1.0639 mL | 2.6598 mL | |
| 40 mM | 0.0798 mL | 0.3990 mL | 0.7979 mL | 1.9948 mL | |
| 50 mM | 0.0638 mL | 0.3192 mL | 0.6383 mL | 1.5959 mL | |
| 60 mM | 0.0532 mL | 0.2660 mL | 0.5320 mL | 1.3299 mL | |
| 80 mM | 0.0399 mL | 0.1995 mL | 0.3990 mL | 0.9974 mL | |
| 100 mM | 0.0319 mL | 0.1596 mL | 0.3192 mL | 0.7979 mL |